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Release info for hum0177

hum0177-v1
2020-04-27
Datasets added in this release
JGAD000280
JGAD000281
JGAD000282
Release note
- PBAT-seq
DNAs extracted from tumor tissues of malignant glioma and cell line were used for PBAT-sequencing analysis (fastq format files). Libraries were prepared with TACS ligation-mediated PBAT (Miura F, Nucleic Acids Res. 2019) and sequenced using Hiseq X Ten (Illumina).
- RNA-seq
RNAs extracted from tumor tissues of malignant glioma and cell line were used for RNA-sequencing analysis (fastq format files). Libraries were prepared with TruSeq stranded mRNA Kit and sequenced using NovaSeq 6000 (Illumina).
- ChIP-seq
DNAs extracted from cell line of malignant glioma were used for ChIP sequencing analysis (fastq format files). Libraries were prepared with SMARTer ThruPLEX DNA-seq Kit. Anti- H3.3 antibody and anti- H3.3 G34V antibody were used for immunoprecipitation. ChIP-Seq libraries were sequenced using NovaSeq 6000 (Illumina).