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We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000282

Type of data
NGS (PBAT-seq, RNA-seq, ChIP-seq)
Access criteria
Controlled-access (Type I)
Total data volume
11.0 GB
File formats
  • FASTQ
Research
hum0177
Date published
2020-09-28
Date modified
2020-09-28
DDBJ Search
JGAD000282 (opens in a new tab)
JGA Study
JGAS000197 (opens in a new tab)

Analysis method

ChIP-seq

Materials and participants
1 Cell line of malignant glioma (ICD10: C71.9)
  • Health status
    Affected
  • Subject count
    1 (Sample)
Disease
  • Cell line of malignant glioma
Sample description
DNAs extracted from a cell line of malignant glioma (1 control [whole cell lysate] and 2 immunoprecipitation samples). Anti- histone H3.3 rabbit monoclonal antibody and anti- histone H3.3 G34V rabbit monoclonal antibody were used for immunoprecipitation.
  • Tumor / normal
    Tumor
Cell line
IFO50356
Sample provider
IFO50356 (JCRB)
Experimental method
ChIP-seq
Target
N/A
Reagent kit
ThruPLEX DNA-Seq Kit
Fragmentation
Ultrasonic fragmentation (Covaris S220)
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Data use policy
NBDC data sharing policy (JGAP000001)