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NBDC HumanDB
JGAD000282
Release date
2020-09-28

Date modified
2020-09-28

Research
hum0177

Type of data
NGS (PBAT-seq, RNA-seq, ChIP-seq)

Access type
Controlled-access (Type I)

JGA Study
JGAS000197

Analysis method

ChIP-seq

Materials and Participants

1 Cell line of malignant glioma (ICD10: C71.9)

Experimental Method

ChIP-seq

Platform

Illumina [NovaSeq 6000]

Sample Description

DNAs extracted from a cell line of malignant glioma (1 control [whole cell lysate] and 2 immunoprecipitation samples). Anti- histone H3.3 rabbit monoclonal antibody and anti- histone H3.3 G34V rabbit monoclonal antibody were used for immunoprecipitation.

Cell Lines

IFO50356 (JCRB)

Library Construction

SMARTer ThruPLEX DNA-seq Kit

Fragmentation

Ultrasonic fragmentation (Covaris S220)

Read Type

Paired-end

Read Length

150 bp

Japanese Genotype-phenotype Archive Dataset Accession
Total Data Volume

1 TB (fastq)

Policies