Aims: Among multifactorial diseases, there are many diseases involving the immune system (e.g., autoimmune diseases, allergic diseases, infectious diseases, cancer, and transplantation) for which there are few appropriate treatments. In order to solve these problems and find better treatments, we believe that a thorough understanding of human immune functions and an appropriate understanding of pathological conditions in these diseases are necessary. Therefore, this study aims to establish a new methodology for disease research based on omics analyses and genetic polymorphism analysis of immune cells such as lymphocytes derived from healthy individuals, and to accumulate important information for understanding pathological conditions and determining targets for drug discovery.

Methods: 5' and 3' single-cell RNA-seq, Micro-C and Region Capture Micro-C, CITE-seq and 5' single-cell RNA-seq, Multimodal assay including single-nucleus ATAC-seq, 3' and 5' single-nucleus RNA-seq, and 5' single-cell RNA-seq, MAS-seq, CAGE-seq, and NET-CAGE-seq were performed on CD4 positive T cells isolated from whole blood of three healthy Japanese. CAGE-seq was performed on CRISPR-activated Jurkat cells.

Targets: Three healthy Japanese (one male, two females), CRISPR-activated Jurkat cells