1) DNAs extracted from peripheral blood cells of 1410 Japanese non-syndromic hearing loss patients were used and 69 gene regions (354 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM/Ion Proton] (Single-end: 200 bp). 2) DNAs extracted from peripheral blood cells of 1559 Japanese non-syndromic hearing loss patients were used and 63 gene regions (295 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM/Ion Proton] (Single-end: 200 bp).

1) DNAs extracted from peripheral blood cells of 3 Japanese non-syndromic hearing loss patients were used and 69 gene regions (354 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM/Ion Proton] (Single-end: 200 bp). 2) DNAs extracted from peripheral blood cells of 11 Japanese non-syndromic hearing loss patients were used and 63 gene regions (295 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM/Ion Proton/Ion S5] (Single-end: 200 bp). 3) DNAs extracted from peripheral blood cells of 1 Japanese non-syndromic hearing loss patients were used and 90 gene regions (568 kb) were targeted to this study. The regions were narrowed down by using of Agilent Sure Select XT and read the sequences by Illumina [HiSeq 2000] (Paired-end: 100 bp).

1) DNAs extracted from peripheral blood cells of 17 Japanese non-syndromic hearing loss patients (JGAS000191: 1 patient, JGAS000192: 9 patients, JGAS000201: 7 patients) were used and 69 gene regions (354 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM/Ion Proton] (Single-end: 200 bp). 2) DNAs extracted from peripheral blood cells of 39 Japanese non-syndromic hearing loss patients (JGAS000192: 17 patients, JGAS000200: 7 patients, JGAS000201: 15 patients) were used and 63 gene regions (295 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM/Ion Proton] (Single-end: 200 bp). 3) DNAs extracted from peripheral blood cells of 6 Japanese non-syndromic hearing loss patients (JGAS000192: 2 patients, JGAS000201: 4 patients) were used and 90 gene regions (568 kb) were targeted to this study. The regions were narrowed down by using of Agilent Sure Select XT and read the sequences by Illumina [HiSeq 2000] (Paired-end: 100 bp).

1) DNAs extracted from peripheral blood cells of 37 Japanese non-syndromic hearing loss patients were used and 69 gene regions (354 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM/Ion Proton] (Single-end: 200 bp). 2) DNAs extracted from peripheral blood cells of 2 Japanese non-syndromic hearing loss patients were used and 90 gene regions (568 kb) were targeted to this study. The regions were narrowed down by using of Agilent Sure Select XT and read the sequences by Illumina [HiSeq 2000] (Paired-end: 100 bp).

1) DNAs extracted from peripheral blood cells of 16 Japanese non-syndromic hearing loss patients were used and 69 gene regions (354 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM/Ion Proton] (Single-end: 200 bp). 2) DNAs extracted from peripheral blood cells of 3 Japanese non-syndromic hearing loss patients were used and 90 gene regions (568 kb) were targeted to this study. The regions were narrowed down by using of Agilent Sure Select XT and read the sequences by Illumina [HiSeq 2000] (Paired-end: 100 bp).

DNAs extracted from peripheral blood cells of 19 Japanese non-syndromic hearing loss patients were used and 69 gene regions (354 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM] or [Ion Proton] (Single-end: 200 bp).

DNAs extracted from peripheral blood cells of 44 Japanese non-syndromic hearing loss patients (JGAS000032: 39 patients, DRA003791: 5 patients) were used and 69 gene regions (354 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit or Ion AmpliSeq Library Kit ver 2.0 and read the sequences by Thermo Fisher Scientific [Ion PGM] or [Ion Proton] (Single-end: 200 bp).

DNAs extracted from peripheral blood cells of 17 Japanese Usher syndrome patients were used and 9 gene regions (109 kb) were targeted to this study. The regions were narrowed down by using of the Ion AmpliSeq Custom kit and read the sequences by Thermo Fisher Scientific [Ion PGM] (Single-end: 200 bp).