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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Research ID

hum0393-v2Release info

Latest

Research title

To explore new biomarkers or therapeutic target molecules using samples with patients with autoimmune diseases

Research overview

Aims
To explore new biomarkers or therapeutic target molecules using samples from patients with autoimmune diseases
Methods
JGAS000693: CD4+CD45RA-CD25hiCD127lo Treg cells were sorted on Moflo XDP cell sorter (Beckman Coulter) from each peripheral blood sample. Total RNA was extracted with TRIZOL-LS (ThermoFisher/Invitrogen) for RNA-seq.
JGAS000630: CD19+cells were separated by magnetic-activated cell sorting (MACS) using human CD19 MicroBeads (Miltenyi Biotec), and then PanT cells were also sorted using human PanT Cell Isolation Kit (Miltenyi Biotec) from each sample. Total RNA was extracted with TRIZOL-LS (ThermoFisher/Invitrogen) for RNA-seq.
Participants/materials
JGAS000693: Twelve peripheral blood samples were collected from six treatment-naïve rheumatoid arthritis (RA) patients and six healthy individuals.
JGAS000630: Submandibular glands (SMGs) (N=3) and peripheral blood mononuclear cells (PBMCs) (N=4) were collected from four patients with treatment naïve IgG4 related diseases (IgG4-RD). PBMCs were also collected from three patients with primary Sjögren's syndrome (pSS) who fulfilled American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) Classification Criteria for pSS, and three healthy controls.
URL
N/A

Datasets

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD000826NGS (RNA-seq)
  • RNA-seq
Controlled-access (Type I)2025-04-10
JGAD000760NGS (RNA-seq)
  • RNA-seq
Controlled-access (Type I)2025-10-29

Data provider

Principal investigator
Hiroto Tsuboi
Affiliation
Department of Rheumatology, University of Tsukuba

Research projects

No research projects.

Grants

NameTitleProject number
KAKENHI Grant-in-Aid for Scientific Research (B)
Mechanisms and exhaustion of T follicular regulatory cells in rheumatoid arthritis
  • 21H02959
establishment of university fellowships towards the creation of science technology innovation, JST
Analysis of age-related changes in regulatory T cell function in rheumatoid arthritis
  • JPMJFS2106
KAKENHI Grant-in-Aid for Scientific Research (C)
Gene expression analysis by RNA-Seq and development of novel therapeutic target in IgG4-related disease
  • 21K08471

Related publications

TitleDOIDataset ID
Mechanisms of age-related Treg dysfunction in an arthritic environment
https://doi.org/10.1016/j.clim.2024.110337 (opens in a new tab)
Analysis of lymphocytes specific gene expression pattern by RNA-Seq in patients with IgG4-related disease: Comparison between submandibular glands and peripheral blood
https://doi.org/10.1093/mr/roaf059 (opens in a new tab)

Controlled access users

Principal investigatorAffiliationCountry/RegionResearch titlePeriod of data useDataset ID
Michiaki HamadaHamada Laboratory, Faculty of Science and Engineering, Waseda UniversityJapanConstruction of RNA-targeted Drug Discovery Database2023-01-05 – 2027-10-31
Takeshi UeharaDepartment of Laboratory Medicine Department of Diagnostic Pathology, Shinshu universityJapanIntegrated analysis of molecular expression profiles in IgG4-related diseases and related conditions2026-02-26 – 2031-01-31