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Dataset ID

JGAD000826

Type of data
NGS (RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
16.5 GB
File formats
  • FASTQ
  • CSV
Research
hum0393
Date published
2025-04-10
Date modified
2025-04-10

Analysis method

RNA-seq

Materials and participants
treatment-naïve rheumatoid arthritis (ICD10: M069): 6 cases (6 samples)
6 healthy individuals (6 samples)
  • Health status
    Mixed
  • Subject count
    22 (Individual)
  • Age group
    Mixed
Disease
treatment-naïve rheumatoid arthritis (M069)
Sample description
RNAs extracted from CD4+CD45RA-CD25hiCD127lo Treg cells sorted on a cell sorter from each peripheral blood sample
  • Tissue
    CD19+ cell, PBMC, Peripheral blood, Submandibular gland, T cell, Treg cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
NEBNext Ultra II DNA Library Prep Kit for Illumina
SMART-Seq
Fragmentation
included in the above library construction kit
Platform
Illumina NextSeq 500
Read type
Paired-end
Read length
36 bp
Reference genome
GRCh37
Mapping
CLC Genomics Workbench
QC and filtering
N/A
Analysis method
CLC Genomics Workbench
Gene count
57,768
Processed data type
TPM