Research ID
hum0358-v1Release info
This page is a past version (v1). The latest version is v4.
Latest version (v4)Research title
Research of genetic abnormalities related to the development and prognosis of adenocarcinoma and squamous cell carcinoma of the cervix and vaginal and vulvar cancer
Research overview
- Aims
- To identify molecular markers involved in the development and prognosis of cervical adenocarcinoma, we would like to elucidate the molecular biological characteristics of cervical adenocarcinoma in relation to the carcinogenesis of other cervical cancers (squamous cell carcinoma), vaginal and vulvar cancers. In addition, some squamous cell carcinomas of the cervix develop at a young age and have a poor prognosis. Furthermore, small cell carcinoma of the uterine cervix also progresses rapidly and has a poor prognosis, making the establishment of diagnostic and therapeutic methods an urgent task.
- Methods
- Whole exome sequencing, RNA-seq, single-cell RNA sequencing, Target bisulfite sequencing
- Participants/materials
- cervical cancer total of 19 + 4 + 96 cases, NIKS18 cell line
[Whole exome sequencing] 1 case: 3 samples of tumor/tumor-derived organoids/peripheral blood cell, 4 cases: 8 tumor samples, 2 peripheral blood samples, 2 normal areas near the tumors, 96 cases: 96 tumor samples
[RNA-seq] 1 case: 2 samples of tumor/tumor-derived organoids, 13 cases: 13 tumor samples, 5 cases: multiple sampling from within the same tumor for a total of 77 samples, 3 cases: 3 samples from normal cervical tissue-derived organoids, 1 case: 4 samples from normal cervical tissue, siRNA-mediated knockdown samples of NIKS18 cell line (siNPM3: 2 samples, siNC: 3 samples)
[single-cell RNA-seq] 1 case: 50cells of normal cervical tissue-derived organoids
[Target bisulfite-seq] 96 cases: 96 tumor samples - URL
- N/A
Datasets
The list is the one this version published; each dataset's content is shown as it is now.
| Cart | Dataset ID | Type of data | Analysis method | Access criteria | Date published |
|---|---|---|---|---|---|
| JGAD000708 | NGS (Exome, RNA-seq) |
| Controlled-access (Type I) | 2022-12-02 | |
| JGAD000713 | NGS (Exome, RNA-seq) |
| Controlled-access (Type I) | 2022-12-21 |
Data provider
- Principal investigator
- Kenbun Sone
- Affiliation
- Department of Obstetrics and Gynecology, The University of Tokyo Hospital
Research projects
No research projects.
Grants
| Name | Title | Project number |
|---|---|---|
Japan Program for Infectious Diseases Research and Infrastructure, Japan Agency for Medical Research and Development (AMED) | Study of HPV-18 persistent infection and carcinogenesis in the squamocolumnar junction cells using novel culture technologies |
|
Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS), Japan Agency for Medical Research and Development (AMED) | Transcriptome analysis of single cell and micro tissue sections for supporting of drug discovery |
|
Japan Program for Infectious Diseases Research and Infrastructure, Japan Agency for Medical Research and Development (AMED) | Elucidation of stem cell carcinogenesis mechanism of HPV18-associated cervical cancer by integration of single-cell analysis technologies Japan Agency for Medical Research and Development |
|
KAKENHI Grant-in-Aid for Early-Career Scientists | Identification of the cell of origin of uterine cervical cancer and elucidation of the regulatory mechanism for carcinogenesis and differentiation |
|
Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS), Japan Agency for Medical Research and Development (AMED) | Support of life science studies by all-in-one single cell/micro tissue multi-omics analysis |
|
Related publications
| Title | DOI | Dataset ID |
|---|---|---|
Cells with stem-like properties are associated with the development of HPV18-positive cervical cancer | ||
Application of organoid culture from HPV18-positive small cell carcinoma of the uterine cervix for precision medicine |
Controlled access users
No use of the controlled access data has been recorded.