Dataset ID
JGAD000708
- Type of data
- NGS (Exome, RNA-seq)
- Access criteria
- Controlled-access (Type I)
- Total data volume
- 76.0 GB
- File formats
- FASTQ
- BAM
- Research
- hum0358
- Date published
- 2022-12-02
- Date modified
- 2022-12-02
- DDBJ Search
- JGAD000708 (opens in a new tab)
- JGA Study
- JGAS000582 (opens in a new tab)
Analysis method
WES
- Materials and participants
- cervical cancer (ICD10: C539)
4 cases: 8 tumor samples, 2 peripheral blood samples, 2 normal areas near the tumor - Health statusAffected
- Subject count15 (Sample)
- Disease
- cervical cancer (C539)
- Sample description
- DNAs extracted from tumors, tumor-derived organoids, peripheral blood cells, or normal areas near the tumor
- TissueOrganoid, Peripheral blood
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- WES
- Target
- N/A
- Reagent kit
- Twist Comprehensive Exome Panel
- Fragmentation
- Ultrasonic fragmentation (Covaris S220)
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- 150 bp
- Reference genome
- GRCh38
- Mapping
- bwa, bowtie2
- Mapping quality
- ≧30
- Coverage (depth)
- 168x
39–318x (Range) - Data use policy
- NBDC data sharing policy (JGAP000001)
RNA-seq
- Materials and participants
- cervical cancer (ICD10: C539)
5 cases: multiple sampling each from within the same tumor for a total of 77 samples - Health statusMixed
- Subject count104 (Sample)
- Disease
- cervical cancer (C539)
- Sample description
- RNAs extracted from tumor tissues, non-tumor tissues, patient-derived organoids, or siRNA-mediated knockdown samples of NIKS18
- TissueOrganoid
- Tumor / normalMixed
- Cell line
- NIKS18
- Sample provider
- NIKS18 is NIKS, which is a human keratinocyte cell line (ATCC CRL-12191), contain HPV18 genome as episomes
- Experimental method
- RNA-seq
- Target
- N/A
- Reagent kit
- Nextera XT DNA Library Prep Kit
- Fragmentation
- Nextera XT transposome
- Platform
- Illumina MiSeq
- Read type
- Paired-end
- Read length
- 75 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)