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Dataset ID

JGAD000713

Type of data
NGS (Exome, RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
25.5 GB
File formats
  • FASTQ
Research
hum0358
Date published
2022-12-21
Date modified
2024-12-09

Analysis method

WES

Materials and participants
cervical cancer (ICD10: C539)
1 case: 3 samples of tumor/tumor-derived organoids/peripheral blood
  • Health status
    Mixed
  • Subject count
    5 (Individual)
Disease
cervical cancer (C539)
Sample description
DNAs extracted from tumors, tumor-derived organoids, peripheral blood cells, or normal areas near the tumor
  • Tissue
    Organoid, Peripheral blood
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WES
Target
N/A
Reagent kit
Twist Comprehensive Exome Panel
Fragmentation
Ultrasonic fragmentation (Covaris S220)
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Reference genome
GRCh38
Mapping
bwa, bowtie2
Mapping quality
≧30
Coverage (depth)
168x
39–318x (Range)

RNA-seq

Materials and participants
cervical cancer (ICD10: C539)
1 case: 2 samples of tumor/tumor-derived organoids
13 cases: 13 samples of tumors
  • Health status
    Mixed
  • Subject count
    23 (Individual)
Disease
cervical cancer (C539)
Sample description
RNAs extracted from tumor tissues, non-tumor tissues, patient-derived organoids, or siRNA-mediated knockdown samples of NIKS18
  • Tissue
    Organoid
  • Tumor / normal
    Mixed
Cell line
NIKS18
Sample provider
NIKS18 is NIKS, which is a human keratinocyte cell line (ATCC CRL-12191), contain HPV18 genome as episomes
Experimental method
RNA-seq
Target
N/A
Reagent kit
Nextera XT DNA Library Prep Kit
Fragmentation
Nextera XT transposome
Platform
Illumina MiSeq
Read type
Paired-end
Read length
75 bp