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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Research ID

hum0611-v1Release info

Latest

Research title

Analysis of Protein and Gene Expression in Blood Cells from Patients with Hematologic Disorders

Research overview

Aims
To analyze protein and gene expression in blood cells obtained from patients with hematologic disorders in order to identify characteristic changes in protein and gene expression associated with specific diseases.
Methods
cDNA libraries were prepared using the SMART-Seq mRNA method (Takara Bio). The amplified double-stranded cDNA was purified using AMPure XP beads (Beckman Coulter). Sequencing libraries were generated using the Nextera XT DNA Library Prep Kit (Illumina) with IDT for Illumina DNA/RNA UD Indexes. Indexed libraries were amplified by PCR and subjected to quality control. Sequencing was performed on the Illumina NovaSeq 6000 platform for the chronic GVHD analysis and on the Illumina NovaSeq X Plus platform for the fisetin analysis, to generate paired-end reads.
Participants/materials
Blood cells obtained from patients with hematologic disorders
1. Analysis according to the presence or absence of chronic GVHD (cGVHD)
cGVHD+ 3 cases and cGVHD– 3 cases
A total of 12 peripheral blood samples: six CD4+ T-cell and six CD8+ T-cell samples.

2. Analysis according to in vitro fisetin treatment
cGVHD+ 1 case
fisetin-treated CD4+ and CD8+ T-cell, untreated CD4+ and CD8+ T-cell samples.
A total of 4 peripheral blood samples.

The participant included in analysis 2 was not included in analysis 1.
URL
N/A

Datasets

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD001144NGS (RNA-seq)
  • RNA-seq
Controlled-access (Type I)2026-11-10

Data provider

Principal investigator
Koji Jimbo
Affiliation
Division of Hematopoietic Disease Control, The Institute of Medical Science, The University of Tokyo

Research projects

No research projects.

Grants

NameTitleProject number
KAKENHI Grant-in-Aid for Early-Career Scientists
Mechanism of T cell senescence after hematopoietic cell transplantation and its impact on chronic graft-versus-host disease
  • 22K16317

Related publications

No related publications.

Controlled access users

No use of the controlled access data has been recorded.