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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Research ID

hum0433-v1Release info

Latest

Research title

Derivation of human trophoblast stem cells and analysis of those characteristics / Derivation of human trophoblast stem cells and those epigenomic landscape

Research overview

Aims
Trophoblast cells play an essential role in the interactions between the fetus and mother, however, the in vitro model to investigate the nature of human trophoblasts has been limited. In this study, we derived human trophoblast stem cells (hTSCs) from first-trimester cytotrophoblasts (CTs) and blastocysts and revealed their epigenomic landscapes including histone modifications, transcription factor binding sites, and long-range chromatin interactions.
Methods
First-trimester placentas are obtained from elective termination of pregnancies. Placenta and blastocysts after delivery are obtained from the normal and hypertensive disorders of pregnancy (HDP) donors. Whole placental villi are enzymatically digested, and CT cells were immunomagnetically purified using a PE-conjugated anti-ITGA6 antibody. CTs were cultured in TS medium containing EGF, CHIR99021, A83-01, VPA, and Y27632. Differentiated and undifferentiated human trophoblast stem cells (hTSCs) are used to investigate mRNA expression, epigenomic features, proliferation, protein expression, and trophoblast functions.
Participants/materials
Placenta after artificial abortion or after delivery and a discarded blastocyst
URL
N/A

Datasets

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD000789NGS (RNA-seq, ChIP-seq, Hi-C)
  • RNA-seq
  • ChIP-seq
  • Hi-C
Controlled-access (Type I)2023-12-26
E-GEAD-665NGS (RNA-seq, ChIP-seq, Hi-C)
  • RNA-seq
  • ChIP-seq
  • Hi-C
Unrestricted-access2023-12-26
JGAD000790NGS (RNA-seq)
  • RNA-seq
Controlled-access (Type I)2024-09-26
E-GEAD-667NGS (RNA-seq)
  • RNA-seq
Unrestricted-access2024-09-26
JGAD000797NGS (RNA-seq, small RNA-seq, CUT&TAG-seq, Methyl-seq)
  • RNA-seq
  • small RNA-seq
  • CUT&TAG-seq
  • Methyl-seq
Controlled-access (Type I)2024-09-26

Data provider

Principal investigator
Eri H Kobayashi
Affiliation
Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine

Research projects

No research projects.

Grants

NameTitleProject number
Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED)
Elucidation of the mechanisms underlying human placental development and design of a placenta-on-a-chip platform
  • JP19gm1310001

Related publications

TitleDOIDataset ID
Derivation of Human Trophoblast Stem Cells
https://doi.org/10.1016/j.stem.2017.11.004 (opens in a new tab)
CRISPR screening in human trophoblast stem cells reveals both shared and distinct aspects of human and mouse placental development
https://doi.org/10.1073/pnas.2311372120 (opens in a new tab)

Controlled access users

No use of the controlled access data has been recorded.