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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000797

Type of data
NGS (RNA-seq, small RNA-seq, CUT&TAG-seq, Methyl-seq)
Access criteria
Controlled-access (Type I)
Total data volume
622 GB
File formats
  • FASTQ
Research
hum0433
Date published
2024-09-26
Date modified
2024-09-26
DDBJ Search
JGAD000797 (opens in a new tab)
JGA Study
JGAS000667 (opens in a new tab)

Analysis method

RNA-seq

Materials and participants
hypertensive disorders of pregnancy (ICD10: O149): 6 cases
CT cells purified from placenta after delivery: 6 samples
normal pregnancy: 3 subjects
CT cells purified from placenta after delivery: 3 samples
  • Health status
    Mixed
  • Subject count
    9 (Individual)
  • Sex
    Female
Disease
hypertensive disorders of pregnancy (O149)
Sample description
RNAs extracted from CT cells
  • Tissue
    Cytotrophoblast, Decidua, Placenta
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
Nextera XT DNA Library Prep Kit
SMART-Seq v4 Ultra Low Input RNA Kit
Fragmentation
included in Nextera XT DNA Library Prep Kit
Platform
Illumina HiSeq 2500
Illumina NovaSeq 6000
Read type
Paired-end
Read length
101 bp
Data use policy
NBDC data sharing policy (JGAP000001)

small RNA-seq

Materials and participants
hypertensive disorders of pregnancy (ICD10: O149): 6 cases
CT cells purified from placenta after delivery: 6 samples
normal pregnancy: 3 subjects
CT cells purified from placenta after delivery: 3 samples
trophoblast stem cells derived from HDP placenta after delivery: 3 samples
  • Health status
    Mixed
  • Subject count
    12 (Mixed)
  • Sex
    Female
Disease
hypertensive disorders of pregnancy (O149)
Sample description
RNAs extracted from CT cells and trophoblast stem cells
  • Tissue
    Cytotrophoblast, Trophoblast stem cell
  • Tumor / normal
    Normal
Cell line
Trophoblast stem cell (TS)
Sample provider
N/A
Experimental method
Small RNA-seq
Target
N/A
Reagent kit
SMARTer smRNA-Seq Kit for Illumina
Fragmentation
N/A
Platform
Illumina NovaSeq 6000
Read type
Single-end
Read length
150 bp
Data use policy
NBDC data sharing policy (JGAP000001)

CUT&TAG-seq

Materials and participants
hypertensive disorders of pregnancy (ICD10: O149): 6 cases
CT cells purified from placenta after delivery: 6 samples x 6 antibodies, 36 samples in total
normal pregnancy: 3 subjects
CT cells purified from placenta after delivery: 3 samples x 6 antibodies, 18 samples in total
  • Health status
    Mixed
  • Subject count
    9 (Individual)
  • Sex
    Female
Disease
hypertensive disorders of pregnancy (O149)
Sample description
DNA fragments purified from CT cells which are incubated with anti-histone antibodies (K4me1, K4me3, K27ac, K36me3, K27m3, K9me3) and pAG-Tn5 transposase
  • Tissue
    Cytotrophoblast
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
CUT&TAG-seq
Target
N/A
Reagent kit
N/A
Fragmentation
Tn5 transposase
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
26 bp
Data use policy
NBDC data sharing policy (JGAP000001)

Methyl-seq

Materials and participants
hypertensive disorders of pregnancy (ICD10: O149): 3 cases
CT cells purified from placenta after delivery: 3 samples
trophoblast stem cells derived from normal placenta after delivery: 2 samples
trophoblast stem cells derived from HDP placenta after delivery: 3 samples
  • Health status
    Mixed
  • Subject count
    8 (Mixed)
  • Sex
    Female
Disease
hypertensive disorders of pregnancy (O149)
Sample description
RNAs extracted from CT cells and trophoblast stem cells
  • Tissue
    Cytotrophoblast, Trophoblast stem cell
  • Tumor / normal
    Normal
Cell line
Trophoblast stem cell (TS)
Sample provider
N/A
Experimental method
Methyl-seq
Target
N/A
Reagent kit
Accel-NGS Methyl-Seq DNA Library Kit
Fragmentation
fragmentation due to bisulfite conversion (EZ DNA Methylation-Gold Kit)
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Data use policy
NBDC data sharing policy (JGAP000001)