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Research ID

hum0427-v2Release info

Latest

Research title

Establishment of cell lines derived from head and neck malignancies / Comprehensive genetic analysis and database construction for head and neck malignancies

Research overview

Aims
- Head and neck malignancies originate in a wide variety of locations, including the pharynx, larynx, oral cavity, nose and paranasal sinuses, salivary glands, thyroid gland, and temporal bone. Their molecular biological characteristics differ depending on the location of origin. Therefore, it is necessary to establish a tailored treatment that is tailored to the molecular biological characteristics of each tumor. In the field of oncology, many researchers generally use cell lines obtained from cell banks. However, cell lines for head and neck malignancies available from cell banks are limited especially for rare-type cancers, resulting in difficulties in elucidation of biological characterization of such malignancies and establishment of therapeutic strategies. This study aimed to 1) conduct comprehensive genetic analysis of head and neck malignant tumor tissues and patient-derived blood, 2) establish cell lines of head and neck malignancies and to examine their tumorigenic abilities in immune-deficient animals, 3) deposit to cell banks. Using multi-omics approaches, we seek to elucidate the molecular mechanisms of oncogenes and to develop biomarkers for recurrence and metastasis prediction, as well as strategies for novel targeted therapies.
Methods
- Genomic DNAs extracted from peripheral blood mononuclear cells (PBMC), the primary tumor tissues, and the cell line were used for whole exome sequencing analysis.
- Chromatin immunoprecipitation (ChIP) sequencing using H3K27Ac and YAP antibodies
Participants/materials
- PBMC and the primary tumor tissues were collected from a patient with external auditory canal squamous cell carcinoma, and a cell line was established from the primary tumor tissues. The cell line was deposited as SCEACono2 in RIKEN BioResource Research Center (https://cellbank.brc.riken.jp/cell_bank/CellInfo/?cellNo=RCB5515&lang=En).
- Five patients with temporal bone squamous cell carcinoma
URL
N/A

Datasets

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD000775NGS (Exome)
  • WES
  • ChIP-seq
Controlled-access (Type I)2023-11-02

Data provider

Principal investigator
Takashi Nakagawa
Affiliation
Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University

Research projects

No research projects.

Grants

NameTitleProject number
KAKENHI Grant-in-Aid for Scientific Research (B)
Development of novel synthesizing treatment strategy by exhaustive research on temporal bone squamous cell carcinoma
  • 18H02951
KAKENHI Grant-in-Aid for Scientific Research (B)
Elucidation of epigenetic tumor control mechanisms that determine the malignancy of squamous cell carcinoma of the temporal bone
  • 22H03236
KAKENHI Grant-in-Aid for Scientific Research (C)
Elucidation of the Metastasis Regulatory Mechanism via NLRP3 Inflammasome Pathway in Temporal Bone Squamous Cell Carcinoma
  • 22K09745
KAKENHI Grant-in-Aid for Early-Career Scientists
Investigation of transcriptional reprogramming mechanisms involved in invasion and metastasis of head and neck squamous cell caricnoma
  • 20K18300
Research Grant, Soda Toyoji Memorial Foundation
Elucidation of Transcriptional Regulatory Mechanisms Contributing to the Progression of Squamous Cell Carcinoma of the External Auditory Canal via Multi-omics Analysis
N/A
Medical Research Grant, Takeda Science Foundation
Elucidation of the YAP1-mediated transcriptional reprogramming mechanism contributing to invasion and metastasis in head and neck squamous cell carcinoma
N/A
Cancer Research Grant, Shinnihon Foundation of Advanced Medical Treatment Research
Exploring Transcriptional Reprogramming Mechanisms Underlying Head and Neck Cancer Progression via Multi-omics Analysis
N/A
Cancer Research Grant, SGH Foundation
Elucidation of Transcriptional Reprogramming Mechanisms involved in Invasion and Metastasis in Head and Neck Squamous Cell Carcinoma
N/A

Related publications

TitleDOIDataset ID
Establishment and characterization of a primary cell culture derived from external auditory canal squamous cell carcinoma
https://doi.org/10.1002/2211-5463.13225 (opens in a new tab)
Aberrant Hippo-YAP/TEAD Signaling Drives Malignant Transcriptional Reprogramming in External Auditory Canal Squamous Cell Carcinoma
https://doi.org/10.1158/2767-9764.crc-25-0626 (opens in a new tab)

Controlled access users

No use of the controlled access data has been recorded.