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Dataset ID

JGAD000775

Type of data
NGS (Exome)
Access criteria
Controlled-access (Type I)
Total data volume
54.5 GB
File formats
  • FASTQ
Research
hum0427
Date published
2023-11-02
Date modified
2026-06-17

Analysis method

WES

Materials and participants
external auditory canal squamous cell carcinoma (ICD10: C44.2): 1 case (3 samples)
non-tumor tissue (PBMC), primary tumor tissue, cell line established from the primary tumor tissue: 1 sample each
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
external auditory canal squamous cell carcinoma (C442)
Sample description
DNAs extracted from PBMC, the primary tumor tissues, and the cell line
  • Tissue
    PBMC, Peripheral blood
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
WES
Target
N/A
Reagent kit
SureSelect Human All Exon V6
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
100 bp

ChIP-seq

Materials and participants
Temporal bone squamous cell carcinoma (ICD10: C44.2): 5 cases
[H3K27Ac]
primary tumor tissues: 5 samples
non-tumor tissues (normal skin): 2 samples
[YAP]
primary tumor tissues: 2 samples
non-tumor tissues (normal skin): 2 samples
Pooled input DNA obtained by mixing DNA from all patients: 1 sample
  • Health status
    Affected
  • Subject count
    5 (Individual)
Disease
Temporal bone squamous cell carcinoma (C442)
Sample description
DNAs extracted from tumor and non-tumor tissues of patients with temporal bone squamous cell carcinoma, and immunoprecipitated with an anti-H3K27Ac and YAP antibody
  • Tissue
    Skin
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
ChIP-seq
Target
N/A
Reagent kit
DNA Library Prep Kit for Illumina
Fragmentation
Ultrasonic fragmentation
Platform
Illumina NextSeq 500
Read type
Single-end
Read length
75 bp