hum0356-v1Release info

Elucidation of genetic alterations in colorectal carcinogenesis and establishment of clinical significance of interstitial

Aims

Recently, single-cell RNA sequencing analysis has been used to analyze cell-cell interactions between colorectal cancer cells and stromal cells in colorectal cancer patients with an adenocarcinoma sequence background. However, the important cancer microenvironment during early cancer development from adenomas, especially the mechanism of immune tolerance acquisition, is unclear. In this study, we investigated immune tolerance and the formation of the tumor microenvironment at the adenoma-cancer interface by integrating single-cell and spatial transcriptome analyses obtained from public databases. By integrating scRNA-seq of colorectal cancer and spatial transcriptome analysis in carcinoma in adenoma tissues, we investigated immune tolerance and tumor microenvironment formation at the adenoma-tumor interface, and thereby searching for new therapeutic target molecules.

Methods

Surgical specimens were frozen in liquid nitrogen. Specimens were cut according to the capture area and sectioned into 10-μm sections using a cryostat. The specimens were placed on Visium Spatial slides and stored at -80 °C until use. Tissues were permeabilized with permeabilization enzyme (10x Genomics: 2000214) for 50 min, washed with 0.1 x SSC buffer (Sigma-Aldrich), and imaged. All steps were performed according to the manufacturer's protocol (CG000240 Rev D, CG000160 Rev A).

Participants/materials

A patient with colorectal cancer with a background of adenocarcinoma sequence

URL

N/A

Principal investigator

Koshi Mimori

Affiliation

Department of Surgery, Kyushu University Beppu Hospital