Aims: DNA extracted from tumor and normal tissue (blood or saliva) was collected from patients with skin tumors. Tumor was primary lesion only (9 cases), metastatic lesion only (5), or both (7). 21 patients with melanoma and 1 patient with sebaceous nevus were enrolled. 29 tumor and 21 normal tissues were analyzed. From the case with sebaceous nevus, DNA was extracted from three parts of a tumor (sebaceous nevus, trichoblastoma and poroma).

Methods: To detect tumor driver mutations, DNAs from tumor and non-tumor tissues were sequenced with a custom panel. Tumor DNA was extracted from patients' formalin-fixed paraffin-embedded sections. The panel contains 3,336 primer-pair for 95 gene regioins.

Targets: 98 patients with melanoma and one patient with sebaceous nevus were enrolled.