Skip to content
NBDC Human Database

No datasets in the cart.

Go to the cart

Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Research ID

hum0273-v1Release info

Latest

Research title

A study of molecular pathology for carcinogenesis and progression of breast cancer

Research overview

Aims
Breast cancer manifests in diverse forms, with particular reference to various cell types harboring different mutations and gene expression profiles. To elucidate the clonal relationship between cancer cells in tumors composed of both ductal and lobular phenotypes, we analyzed two combined lobular and ductal carcinoma cases by next generation sequencing.
Methods
DNA from samples were used for amplicon sequencing by an Ion AmpliSeq Comprehensive Cancer Panel on an Ion Torrent platform (Thermo Fisher SCIENTIFIC). The panel consists of approximately 16,000 primer pairs covering all exons of 409 cancer-associated genes (1.6 megabases of target sequences). DNA (120 ng) was used to prepare the libraries with IonXpress barcoded adapters. An Ion Proton Sequencer (Thermo Fisher SCIENTIFIC) was used for sequencing with the ion chip.
Participants/materials
A total of 9 DNAs collected by laser microdissection from 2 cases of lobular carcinoma in situ with ductal carcinoma in situ.
URL
N/A

Datasets

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD000411NGS (Amplicon-seq)
  • RNA Amplicon-seq
Controlled-access (Type I)2021-08-18

Data provider

Principal investigator
Shinobu Masuda
Affiliation
Division of Oncologic Pathology, Department of Pathology and Microbiology, Nihon University School of Medicine

Research projects

No research projects.

Grants

NameTitleProject number
KAKENHI Grant-in-Aid for Scientific Research (C)
A cell lineage of breast cancer consisting of different histologic types by mutation analysis of mtDNA D-loop region
  • 15K08357

Related publications

TitleDOIDataset ID
Phylogenetic analysis of combined lobular and ductal carcinoma of the breast
https://doi.org/10.3892/mmr.2021.12357 (opens in a new tab)

Controlled access users

No use of the controlled access data has been recorded.