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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Research ID

hum0266-v1Release info

Latest

Research title

Elucidation of the pathogenesis of autoimmune dermatosis using patient samples.

Research overview

Aims
Pemphigus, an autoimmune skin disease, is caused by the production of autoantibodies against desmoglein, an autoantigen. Clinically, treatment targeting B cells has been shown to be effective, and desmoglein-specific B cells may play an important role in the pathogenesis. The purpose of this research is to isolate desmoglein-specific B cells in the peripheral blood of patients using labeled desmoglein protein, and to analyze their characteristics and changes in gene expression due to treatment by comprehensive genetic analysis, and to link them to disease markers and treatments.
Methods
Antigen-specific B cells from PBMCs were single-cell sorted with FACS ARIA3 using labeled desmoglein protein or influenza HA antigen, libraries were prepared with SMART-seq, and sequenced with Novaseq.
Participants/materials
Desmoglein-specific B cells and non-desmoglein-specific B cells from 3 patients with pemphigus
One of the patients, desmoglein-specific B cells and non-desmoglein-specific B cells after PSL treatment are also included.
Influenza HA specific B cells and non-influenza HA specific B cells from 3 healthy subjects as controls
URL
N/A

Datasets

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD000387NGS (scRNA-seq)
  • scRNA-seq
Controlled-access (Type I)2022-12-28

Data provider

Principal investigator
Shohei Egami
Affiliation
Department of Dermatology, Keio University School of Medicine

Research projects

No research projects.

Grants

NameTitleProject number
KAKENHI Grant-in-Aid for Scientific Research (B)
Elucidating pathophysiology of pemphigus by single cell analysis of auto-reactive B cells
  • 19H03683
Health and Labor Sciences Research Grants for Research on Rare and Intractable Diseases
Research on rare intractable skin diseases
  • 20FC1052

Related publications

No related publications.

Controlled access users

Principal investigatorAffiliationCountry/RegionResearch titlePeriod of data useDataset ID
Michiaki HamadaHamada Laboratory, Faculty of Science and Engineering, Waseda UniversityJapanConstruction of RNA-targeted Drug Discovery Database2023-01-05 – 2027-10-31
Seonpil JinDermatology, Seoul National University HospitalSouth KoreaThe Study of Immunologic Mechanisms of Tertiary Lymphoid Structures in Pemphigus Using Spatial Transcriptomics2026-01-15 – 2027-11-10