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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000387

Type of data
NGS (scRNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
196 GB
File formats
  • FASTQ
  • TXT
Research
hum0266
Date published
2022-12-28
Date modified
2022-12-28
DDBJ Search
JGAD000387 (opens in a new tab)
JGA Study
JGAS000281 (opens in a new tab)

Analysis method

scRNA-seq

Materials and participants
Pemphigus (ICD10: L10.0/L10.2): 3 cases (number of single cells)
Desmoglein-specific B cell (37 , 30 , 36)
non-desmoglein-specific B cell (44 , 40 , 44)
One of the patients, desmoglein-specific B cells and non-desmoglein-specific B cells (31 , 96) after PSL treatment are also included.
3 healthy controls (number of single cells)
Influenza HA specific B cell (48 , 36 , 36)
non-influenza HA specific B cell (48 , 48 , 48)
  • Health status
    Mixed
  • Subject count
    6 (Individual)
Disease
Pemphigus (L100, L102)
Sample description
RNAs extracted from antigen-specific B cells
  • Tissue
    B cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Nextera XT DNA Library Prep Kit
SMART-Seq HT Kit
Fragmentation
Nextera XT DNA Library Prep Kit
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Data use policy
NBDC data sharing policy (JGAP000001)