hum0130-v1Release info

Comprehensive genetic analyses of bone marrow cells in POEMS syndrome / Analyses of molecular mechanisms of hematological malignancies and bone marrow niche

Aims

To compare genetic features of plasma cells from polyneuropathy, organomegaly, endocrinopathy, M-Protein, and skin changes (POEMS) syndrome, multiple myeloma (MM), and monoclonal gammopathy of undetermined significance (MGUS), we herein performed WES, target sequencing, bulk RNA-seq, single-cell RNA Seq of bone marrow (BM) plasma cells from patients with POEMS syndrome and compared them with other diseases and normal controls. These data unveil unique features of POEMS clones among plasma cell neoplasms and enhance our understanding of the pathogenesis of POEMS syndrome.

Methods

Genomic analysis of POEMS syndrome, and gene expression analysis of 3 diseases. For scRNA-Seq, CD138+ plasma cells were purified by FACS after MACS CD138 positive selection from BM aspiration samples. Single cell isolation and library preparation were performed by Fluidigm C1, and then the samples were read by single-end sequencing with Illumina HiSeq 3000. For bulk RNA-Seq, library preparation was performed by SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing and NEBNext Ultra DNA Library Prep Kit. Then the samples were read by paired-end sequencing with Illumina HiSeq 2000. RAN-seq was performed on bone marrow plasma cells purified by fluorescence-activated cell sorting (FACS) after CD138-positive magnetic cell separation. RNA samples were obtained from 3 early-stage lymphoma patients without BM invasion as normal controls.

Participants/materials

Plasma cells from POEMS syndrome, MM, MGUS, and Normal control (lymphoma without BM invasion)

URL

N/A

Principal investigator

Emiko Sakaida

Affiliation

Chiba University Graduate school of Medicine, Clinical Cell Bilogy and Medicine

No research projects.