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NBDC Human Database

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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Release info for hum0086

  • hum0086-v3

    2018-06-26
    Datasets added in this release

    No datasets were added.

    Release note
    Data addition of - RNA-seq data for placental stroma - PBAT-seq, RNA-seq, and small RNA-seq data for cytotrophoblast (fetal growth restriction, preeclampsia, trisomy 18) - PBAT-seq data for hydatidiform mole hum0086.v2 Data addition of - PBAT-seq, ChIP-seq, RNA-seq, and small RNA-seq data for female trophoblast stem cells (TS) derived from CT, ST differentiated from TS, EVT differentiated from TS - PBAT-seq data for male TS derived from CT - RNA-seq data for male TS derived from CT, ST differentiated from TS, and EVT differentiated from TS - ChIP-seq data for placental stroma hum0086.v1 NGS (PBAT-seq) DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for PBAT-seq analyses (fastq format files). Libraries were prepared by Post-Bisulfite Adaptor-Tagging (PBAT) method and sequencing was performed with Illumina [HiSeq 1500 / 2500] (101 bp, single-end / paired-end). NGS (ChIP-seq) DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for ChIP-seq analyses (fastq format files). Anti H3K4me3, H3K4me1, H3K9me3, H3K27ac, H3K27me3, H3K36me3 antibodies were used for immunoprecipitation. ChIP-Seq libraries were prepared by using Ovation Ultralow System V2 kit (NuGEN) and NEBNext UltraII DNA Library Prep Kit for Illumina and sequenced using Illumina [HiSeq 2500] (51 - 126 bp, single-end / paired-end). NGS (RNA-seq) mRNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for RNA-seq analyses (fastq format files). Libraries were prepared by using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and sequencing was performed with Illumina [HiSeq 2500] (101-126 bp, paired-end). NGS (small RNA-seq) RNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for small RNA-seq analyses (fastq format files). Libraries were prepared by using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) and sequencing was performed with Illumina [HiSeq 1500 / 2500] (64 / 68 bp [including adaptor], single-end).
  • hum0086-v2

    2017-12-26
    Datasets added in this release
    Release note
  • hum0086-v1

    2017-09-25
    Datasets added in this release
    Release note