We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.
hum0086-v3
Datasets added in this release
No datasets added in this release.
Release note
Data addition of - RNA-seq data for placental stroma - PBAT-seq, RNA-seq, and small RNA-seq data for cytotrophoblast (fetal growth restriction, preeclampsia, trisomy 18) - PBAT-seq data for hydatidiform mole hum0086.v2 Data addition of - PBAT-seq, ChIP-seq, RNA-seq, and small RNA-seq data for female trophoblast stem cells (TS) derived from CT, ST differentiated from TS, EVT differentiated from TS - PBAT-seq data for male TS derived from CT - RNA-seq data for male TS derived from CT, ST differentiated from TS, and EVT differentiated from TS - ChIP-seq data for placental stroma hum0086.v1 NGS (PBAT-seq) DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for PBAT-seq analyses (fastq format files). Libraries were prepared by Post-Bisulfite Adaptor-Tagging (PBAT) method and sequencing was performed with Illumina [HiSeq 1500 / 2500] (101 bp, single-end / paired-end). NGS (ChIP-seq) DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for ChIP-seq analyses (fastq format files). Anti H3K4me3, H3K4me1, H3K9me3, H3K27ac, H3K27me3, H3K36me3 antibodies were used for immunoprecipitation. ChIP-Seq libraries were prepared by using Ovation Ultralow System V2 kit (NuGEN) and NEBNext UltraII DNA Library Prep Kit for Illumina and sequenced using Illumina [HiSeq 2500] (51 - 126 bp, single-end / paired-end). NGS (RNA-seq) mRNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for RNA-seq analyses (fastq format files). Libraries were prepared by using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and sequencing was performed with Illumina [HiSeq 2500] (101-126 bp, paired-end). NGS (small RNA-seq) RNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for small RNA-seq analyses (fastq format files). Libraries were prepared by using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) and sequencing was performed with Illumina [HiSeq 1500 / 2500] (64 / 68 bp [including adaptor], single-end).
Vdersion release date
2018-06-26hum0086-v2
Datasets added in this release
Release note
Vdersion release date
2017-12-26hum0086-v1
Datasets added in this release
Release note
Vdersion release date
2017-09-25
