Release info for hum0086
hum0086-v3
2018-06-26- Datasets added in this release
No datasets were added.
- Release note
- Data addition of - RNA-seq data for placental stroma - PBAT-seq, RNA-seq, and small RNA-seq data for cytotrophoblast (fetal growth restriction, preeclampsia, trisomy 18) - PBAT-seq data for hydatidiform mole hum0086.v2 Data addition of - PBAT-seq, ChIP-seq, RNA-seq, and small RNA-seq data for female trophoblast stem cells (TS) derived from CT, ST differentiated from TS, EVT differentiated from TS - PBAT-seq data for male TS derived from CT - RNA-seq data for male TS derived from CT, ST differentiated from TS, and EVT differentiated from TS - ChIP-seq data for placental stroma hum0086.v1 NGS (PBAT-seq) DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for PBAT-seq analyses (fastq format files). Libraries were prepared by Post-Bisulfite Adaptor-Tagging (PBAT) method and sequencing was performed with Illumina [HiSeq 1500 / 2500] (101 bp, single-end / paired-end). NGS (ChIP-seq) DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for ChIP-seq analyses (fastq format files). Anti H3K4me3, H3K4me1, H3K9me3, H3K27ac, H3K27me3, H3K36me3 antibodies were used for immunoprecipitation. ChIP-Seq libraries were prepared by using Ovation Ultralow System V2 kit (NuGEN) and NEBNext UltraII DNA Library Prep Kit for Illumina and sequenced using Illumina [HiSeq 2500] (51 - 126 bp, single-end / paired-end). NGS (RNA-seq) mRNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for RNA-seq analyses (fastq format files). Libraries were prepared by using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and sequencing was performed with Illumina [HiSeq 2500] (101-126 bp, paired-end). NGS (small RNA-seq) RNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for small RNA-seq analyses (fastq format files). Libraries were prepared by using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) and sequencing was performed with Illumina [HiSeq 1500 / 2500] (64 / 68 bp [including adaptor], single-end).
hum0086-v2
2017-12-26- Datasets added in this release
- Release note
hum0086-v1
2017-09-25- Datasets added in this release
- Release note