Dataset ID

NHA000190

Name: NHA000190: GWAS for gut microbiome, GWAS for plasma metabolite, GWAS for KEGG Gene Ortholog and KEGG Pathway
Published: 2023-10-02

Type of data: GWAS for gut microbiome
GWAS for plasma metabolite
GWAS for KEGG Gene Ortholog and KEGG Pathway
Access criteria: Unrestricted-access
Total data volume: 320 GB
File formats: TSV
DOCX
Research: hum0197
Date published: 2023-10-02
Date modified: 2023-10-02
Secondary ID: hum0197.v18.gwas.v1

Unrestricted-access files linked to this dataset

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301–320 / 732

File	Label	Size
metabo_P_0150_QCed_sumstats.tsv.gz	Acylcarnitine(20:1)-2	319 MB
metabo_P_0151_QCed_sumstats.tsv.gz	Acylcarnitine(20:0)	318 MB
metabo_P_0157_QCed_sumstats.tsv.gz	α-Tocopherol acetate	318 MB
metabo_P_0167_QCed_sumstats.tsv.gz	Astaxanthin	319 MB
metabo_P_0170_QCed_sumstats.tsv.gz	Trilaurin-2	319 MB
metabo_P_0171_QCed_sumstats.tsv.gz	1,2-Dipalmitoyl-glycero-3-phosphoethanolamine	318 MB
metabo_P_0172_QCed_sumstats.tsv.gz	Sphingomyelin(d18:1/16:0)	318 MB
metabo_P_0174_QCed_sumstats.tsv.gz	Thyroxine	318 MB
metabo_P_0175_QCed_sumstats.tsv.gz	1,2-Distearoyl-glycero-3-phosphocholine-1	318 MB
microbiome_L7_species_Absiella_dolichum_QCed_sumstats.tsv.gz	Absiella_dolichum	523 MB
microbiome_L7_species_Absiella_sp_QCed_sumstats.tsv.gz	Absiella_sp	521 MB
microbiome_L7_species_Acetivibrio_sp_QCed_sumstats.tsv.gz	Acetivibrio_sp	522 MB
microbiome_L7_species_Acidaminococcus_fermentans_QCed_sumstats.tsv.gz	Acidaminococcus_fermentans	526 MB
microbiome_L7_species_Acidaminococcus_intestini_QCed_sumstats.tsv.gz	Acidaminococcus_intestini	526 MB
microbiome_L7_species_Acidaminococcus_massiliensis_QCed_sumstats.tsv.gz	Acidaminococcus_massiliensis	526 MB
microbiome_L7_species_Acidaminococcus_sp_QCed_sumstats.tsv.gz	Acidaminococcus_sp	524 MB
microbiome_L7_species_Acinetobacter_baumannii_QCed_sumstats.tsv.gz	Acinetobacter_baumannii	525 MB
microbiome_L7_species_Acinetobacter_sp_QCed_sumstats.tsv.gz	Acinetobacter_sp	522 MB
microbiome_L7_species_Actinobaculum_sp_QCed_sumstats.tsv.gz	Actinobaculum_sp	522 MB
microbiome_L7_species_Actinomyces_dentalis_QCed_sumstats.tsv.gz	Actinomyces_dentalis	525 MB

301–320 / 732

Analysis method

genome wide SNPs

Materials and participants: 524 Japanese individuals (423 species in the gut microbiome)
306 Japanese individuals (306 plasma metabolites)
524 Japanese individuals (KEGG Gene Ortholog and KEGG Pathway); Subject count
524 (Individual)
Population
Japanese
Sample description: DNAs extracted from peripheral blood cells; Tissue
Peripheral blood
Tumor / normal
Normal
Experimental method: Genotyping by array
WGS
Reagent kit: Infinium Asian Screening Array Kit
KAPA Hyper Prep Kit
TruSeq DNA PCR-Free Library Prep Kit
Platform: Illumina HiSeq 2500
Illumina HiSeq 3000
Illumina HiSeq X
Illumina Infinium Asian Screening Array
Illumina NovaSeq 6000
Reference genome: GRCh37
QC and filtering: SNP array data:
Sample QC: We excluded individuals with low genotyping call rates (call rate < 98%). We included individuals of the estimated Asian ancestry using PCA.
Variant QC: We excluded variants with (1) genotyping call rate < 99%, (2) minor allele count < 5, (3) P-value for Hardy-Weinberg equilibrium < 1.0 × 10^−10, and (4) > 5% allele frequency difference compared with the imputation reference panel or the allele frequency panel of Tohoku Medical Megabank Project.
Post-imputation QC: We excluded imputed variants with Rsq < 0.7 and minor allele frequency < 1%.
WGS:
We excluded variants with genotype call rate <90%, ExcessHet > 60, Hardy-Weinberg P<1.0×10−10
After imputation with Beagle v5.1, we excluded imputed variants with minor allele frequency < 1%.
Imputation: Haplotype phasing: shapeit4
Imputation: minimac4
Analysis method: SNP array:
Genotyping: GenomeStudio
WGS:
WA-MEM v0.7.13 + GATK v3.8-0
PLINK2
Variant count: Gut microbiota/KEGG (SNP array): 7,213,470 variants
Blood metabolites (WGS): 6,840,258 variants
Processed data type: GWAS summary statistics
Phenotype data: Included
Data use policy: NBDC data sharing policy (JGAP000001)