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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
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Dataset ID

NHA000066

Type of data
(3) Average of FPKM values from RNA-seq
Access criteria
Unrestricted-access
Total data volume
874 KB
File formats
  • XLSX
  • ZIP
Research
hum0056
Date published
2018-03-30
Date modified
2018-03-30
Secondary ID
hum0056.v1.fpkm.v1

Unrestricted-access files linked to this dataset

Download the URL list
FileLabelSizeCopy URL
hum0056.v1.fpkm.v1.zip860 KB
hum0056_header.xlsxDictionary file13.3 KB

Analysis method

RNA-seq

Materials and participants
IMM cohort: 197 individuals
  • Subject count
    197 (Individual)
Sample description
RNAs extracted from 102 monocytes, 102 CD4+ T cells, and 94 neutrophils
  • Tissue
    CD4+ T cell, Monocyte, Neutrophil
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
TruSeq RNA Library Prep Kit
Fragmentation
divalent cation under high temperature
Platform
Illumina HiSeq 2500
Read type
Single-end
Read length
125 bp
Reference genome
GRCh37
Mapping
TopHat (ver. 2.0.13)
Read deduplication
none
Realignment and base quality recalibration
none
QC and filtering
electrophoresis and qPCR
Analysis method
cuffquant and cuffnorm
Read count
Monocytes: 33,917,157 ± 3,153,528 / 27,390,039 ± 2,494,286
CD4+ T cells: 35,175,996 ± 1,275,575 / 27,506,624 ± 1,669,459
Neutrophils: 47,040,140 ± 6,289,540 / 43,241,139 ± 8,491,065
Coverage (depth)
N/A
Coverage (breadth)
N/A
Gene count
Monocytes: 16,282 genes
CD4-positive T lymphocytes: 18,299 genes
Neutrophils: 14,534 genes
Processed data type
FPKM
Data use policy
NBDC data sharing policy (JGAP000001)