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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD001063

Type of data
NGS (RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
12.1 GB
File formats
  • FASTQ
  • TXT
Research
hum0574
Date published
2026-07-27
Date modified
2026-07-27
DDBJ Search
JGAD001063 (opens in a new tab)
JGA Study
JGAS000919 (opens in a new tab)

Analysis method

RNA-seq

Materials and participants
rectal cancer (ICD10: C20): 2 cases (one with POLE mutation-positive rectal cancer and one with POLE mutation-negative rectal cancer), 4 samples in total
tumor tissue (a pre-treatment tumor biopsy and a tumor biopsy during chemoradiotherapy): 2 samples each
  • Health status
    Affected
  • Subject count
    2 (Individual)
  • Population
    Japanese
Disease
rectal cancer (C20)
Sample description
RNA extracted from tumor tissues
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
TruSeq Stranded mRNA Library Prep Kit
Fragmentation
Heat treatment
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Reference genome
GRCh37
Mapping
TopHat v2.0.13
QC and filtering
Cutadapt v1.2.1 and PRINSEQ v0.19.2
Analysis method
Cuffquant / Cufflinks package v2.2.1
Gene count
13,000
Data use policy
NBDC data sharing policy (JGAP000001)