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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000915

Type of data
NGS (scRNA-seq/TCR/BCR repertoire)
NGS (Visium Spatial Gene Expression), Histological image
Access criteria
Controlled-access (Type I)
Total data volume
1.2 TB
File formats
  • FASTQ
  • BAM
  • CSV
  • JPEG
  • TAR
Research
hum0492
Date published
2025-08-13
Date modified
2025-12-11
DDBJ Search
JGAD000915 (opens in a new tab)
JGA Study
JGAS000773 (opens in a new tab)

Analysis method

scRNA-seq + scTCR-seq + scBCR-seq

Materials and participants
Sjögren's syndrome (ICD10: M350): 19 cases
Sicca: 8 cases
labial salivary glands: total 27 samples
(6 patients with Sjögren's syndrome and 3 patients with Sicca are also used in Visium analysis)
  • Health status
    Affected
  • Subject count
    27 (Individual)
Disease
Sjögren's syndrome (M350)
Sample description
RNAs extracted from T cell or B cell obtained from labial salivary glands
  • Tissue
    Salivary gland
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
BCR-seq
scRNA-seq
TCR-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 5' v1.1
Fragmentation
included in the above library construction kit
Platform
Illumina HiSeq X
Read type
Paired-end
Read length
150 bp
Reference genome
GRCh38
Mapping
Cell Ranger (version 5.0.1)
QC and filtering
Cell Ranger (version 5.0.1)
Analysis method
Cell Ranger (version 5.0.1)
Gene count
1478 (Mean)
Data use policy
NBDC data sharing policy (JGAP000001)

Visium 空間トランスクリプトーム + 病理画像

Materials and participants
Sjögren's syndrome (ICD10: M350): 15 cases
Sicca: 5 cases
labial salivary glands: total 20 samples
(6 patients with Sjögren's syndrome and 3 patients with Sicca are also used in scRNA-seq/TCR/BCR repertoire analysis)
  • Health status
    Affected
  • Subject count
    20 (Individual)
Disease
Sjögren's syndrome (M350)
Sample description
labial salivary glands
  • Tissue
    Salivary gland
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
Histological image
Spatial transcriptomics
Target
N/A
Sample processing
Histological staining: hematoxylin-eosin staining
Reagent kit
Visium CytAssist Spatial Gene Expression for FFPE
Fragmentation
included in the above library construction kit
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
read1: 28 bp
read2: 50 bp
Reference genome
GRCh38
Analysis method
spaceranger-2.0.1
Phenotype data
Included
Data use policy
NBDC data sharing policy (JGAP000001)