Dataset ID
JGAD000704
- Type of data
- NGS (scRNA-seq, scVDJ-seq)
- Access criteria
- Controlled-access (Type I)
- Total data volume
- 511 GB
- File formats
- FASTQ
- TSV
- Research
- hum0379
- Date published
- 2023-11-20
- Date modified
- 2023-11-20
- DDBJ Search
- JGAD000704 (opens in a new tab)
- JGA Study
- JGAS000578 (opens in a new tab)
Analysis method
scRNA-seq + scVDJ-seq
- Materials and participants
- MG (ICD10: G70.0): 2 cases (CD4+ T cell and B cell specimens each)
MS (ICD10: G35): 4 cases (3 cases of CD4+ T cell and B cell specimens each + 1 mixed CD4+ T cell and B cell specimen)
SLE (ICD10: M32.9): 3 cases (3 cases of CD4+ T cell and B cell specimens each + 1 CD4+ T cell pecimen)
3 healthy cases (CD4+ T cell and B cell specimens each) - Health statusMixed
- Subject count12 (Individual)
- Disease
- MG (G700)
MS (G35)
SLE (M329) - Sample description
- RNAs extracted from CD4+ T cell and B cell isolated from peripheral blood
- TissueB cell, CD4+ T cell
- Tumor / normalNormal
- Sample provider
- N/A
- Experimental method
- scRNA-seq
VDJ-seq - Target
- N/A
- Reagent kit
- Chromium Next GEM Single Cell 5' v2
Chromium Single Cell Human BCR Amplification Kit
Chromium Single Cell Human TCR Amplification Kit - Fragmentation
- included in the above library construction kit
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- 91 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)