Dataset ID
JGAD000696
- Type of data
- NGS (WGS: NovaSeq 6000)
NGS (WGS: PromethION)
NGS (RNA-seq : NovaSeq 6000, HiSeq 2000)
NGS (Target Capture)
NGS (Visium Spatial Gene Expression), Histological image
Multiplex Fluorescence Immunostaining - Access criteria
- Controlled-access (Type I)
- Total data volume
- 24.0 TB
- File formats
- FASTQ
- TSV
- CSV
- XLSX
- JSON
- MTX
- PNG
- QPTIFF
- TAR
- Research
- hum0068
- Date published
- 2023-02-10
- Date modified
- 2026-09-26
- DDBJ Search
- JGAD000696 (opens in a new tab)
- JGA Study
- JGAS000570 (opens in a new tab)
Unrestricted-access files linked to this dataset
| File | Label | Size | Copy URL |
|---|---|---|---|
| cycle_ | cycle/probe information for JGAD000696 | 191 KB | |
| gene_ | 302 genes | 153 KB |
Analysis method
WGS
- Materials and participants
- lung adenocarcinoma (ICD10: C349): 21 + 56 cases (42 + 112 samples)
tumor tissues: 21 + 56 samples
normal tissues: 21 + 56 samples - Health statusAffected
- Subject count77 (Individual)
- Disease
- lung adenocarcinoma (C349)
- Sample description
- DNAs extracted from tumor and normal tissues of lung adenocarcinomas
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- WGS
- Target
- N/A
- Reagent kit
- TruSeq DNA PCR-Free Library Prep Kit
TruSeq Nano DNA Library Prep Kit - Fragmentation
- Ultrasonic fragmentation (Covaris)
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- N/A
- Dataset ID of processed data
- JGAD000670
- Processing method
- Whole Genome Sequencing Analysis (germline)
- Data use policy
- NBDC data sharing policy (JGAP000001)
WGS
- Materials and participants
- lung adenocarcinoma (ICD10: C349): 21 + 42 cases (41 + 8 + 81 samples)
tumor tissues: 21 + 4 + 42 samples
normal tissues: 20 + 4 + 39 samples - Health statusAffected
- Subject count63 (Individual)
- Disease
- lung adenocarcinoma (C349)
- Sample description
- DNAs extracted from tumor and normal tissues of lung adenocarcinomas
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- WGS
- Target
- N/A
- Reagent kit
- Ligation Sequencing Kit (SQK-LSK109)
- Fragmentation
- N/A
- Platform
- Oxford Nanopore Technologies PromethION
- Read type
- Single-end
- Read length
- N/A
- Data use policy
- NBDC data sharing policy (JGAP000001)
RNA-seq
- Materials and participants
- lung adenocarcinoma (ICD10: C349): 6 + 5 + 26 cases (12 + 5 + 26 samples)
tumor tissues: 6 + 5 + 26 samples
normal tissues: 6 samples - Health statusAffected
- Subject count37 (Individual)
- Disease
- lung adenocarcinoma (C349)
- Sample description
- RNAs extracted from tumor and normal tissues of lung adenocarcinomas
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- RNA-seq
- Target
- N/A
- Reagent kit
- Nextera XT DNA Library Prep Kit
SMART-Seq Stranded Kit
SMART-Seq v4 Ultra Low Input RNA Kit
TruSeq Stranded mRNA Library Prep Kit
TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold - Fragmentation
- Tagmentation (Nextera XT DNA Library Preparation Kit)
- Platform
- Illumina HiSeq 2000
Illumina NovaSeq 6000 - Read type
- Paired-end
- Read length
- 300 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)
Targeted DNA sequencing
- Materials and participants
- lung adenocarcinoma (ICD10: C349): 36 cases
tumor tissues: 36 samples - Health statusAffected
- Subject count36 (Individual)
- Disease
- lung adenocarcinoma (C349)
- Sample description
- DNAs extracted from tumor tissues of lung adenocarcinomas
- Tumor / normalTumor
- Sample provider
- N/A
- Experimental method
- Targeted DNA sequencing
- Target
- NCC Oncopanel v2
https://www.ncc.go.jp/en/information/press_release/20190717/20190717152024.html - Reagent kit
- NCC Oncopanel
- Fragmentation
- N/A
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- 300 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)
Multiplex Fluorescence Immunostaining
- Materials and participants
- lung adenocarcinoma (ICD10: C349): 3 + 6 cases
tumor tissues: 3 + 6 samples - Health statusAffected
- Subject count9 (Individual)
- Disease
- lung adenocarcinoma (C349)
- Sample description
- tumor tissues of lung adenocarcinomas
- Tumor / normalTumor
- Sample provider
- N/A
- Experimental method
- Multiplex fluorescence immunostaining
- Target
- cycle/probe information
- Sample processing
- Cell treatment: Staining Kit for PhenoCycler
- Platform
- Akoya Biosciences PhenoCycler
- Data use policy
- NBDC data sharing policy (JGAP000001)
Xenium 空間トランスクリプトーム
- Materials and participants
- lung adenocarcinoma (ICD10: C349): 1 + 1 + 10 cases
tumor tissues: 1 + 1 + 10 samples - Health statusAffected
- Subject count12 (Individual)
- Disease
- lung adenocarcinoma (C349)
- Sample description
- tumor tissues of lung adenocarcinomas
- Tumor / normalTumor
- Sample provider
- N/A
- Experimental method
- Spatial transcriptomics
- Target
- 302 genes (Human Lung panel: 202 genes, custom: 100 genes)
- Reagent kit
- Xenium Decoding Consumables
Xenium Decoding Reagents
Xenium Slides & Sample Prep Reagents - Platform
- 10x Genomics Xenium Analyzer
- Analysis method
- xenium-1.1.0.2
xenium-1.5.1.2 - Data use policy
- NBDC data sharing policy (JGAP000001)
Visium 空間トランスクリプトーム + 病理画像
- Materials and participants
- lung adenocarcinoma (ICD10: C349): 3 + 19 cases
tumor tissues: 3 + 25 samples - Health statusAffected
- Subject count22 (Individual)
- Disease
- lung adenocarcinoma (C349)
- Sample description
- RNAs extracted from tumor tissues of lung adenocarcinomas
- Tumor / normalTumor
- Sample provider
- N/A
- Experimental method
- Histological image
Spatial transcriptomics - Target
- N/A
- Reagent kit
- Visium Spatial Gene Expression
- Fragmentation
- N/A
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- 118 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)