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Dataset ID

JGAD000696

Type of data
NGS (WGS: NovaSeq 6000)
NGS (WGS: PromethION)
NGS (RNA-seq : NovaSeq 6000, HiSeq 2000)
NGS (Target Capture)
NGS (Visium Spatial Gene Expression), Histological image
Multiplex Fluorescence Immunostaining
Access criteria
Controlled-access (Type I)
Total data volume
24.0 TB
File formats
  • FASTQ
  • TSV
  • CSV
  • XLSX
  • JSON
  • MTX
  • PNG
  • QPTIFF
  • TAR
Research
hum0068
Date published
2023-02-10
Date modified
2026-09-26

Unrestricted-access files linked to this dataset

FileLabelSizeCopy URL
cycle_probe_info_JGAD000696.pdfcycle/probe information for JGAD000696191 KB
gene_list_302.pdf302 genes153 KB

Analysis method

WGS

Materials and participants
lung adenocarcinoma (ICD10: C349): 21 + 56 cases (42 + 112 samples)
tumor tissues: 21 + 56 samples
normal tissues: 21 + 56 samples
  • Health status
    Affected
  • Subject count
    77 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
DNAs extracted from tumor and normal tissues of lung adenocarcinomas
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WGS
Target
N/A
Reagent kit
TruSeq DNA PCR-Free Library Prep Kit
TruSeq Nano DNA Library Prep Kit
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
N/A
Dataset ID of processed data
JGAD000670

WGS

Materials and participants
lung adenocarcinoma (ICD10: C349): 21 + 42 cases (41 + 8 + 81 samples)
tumor tissues: 21 + 4 + 42 samples
normal tissues: 20 + 4 + 39 samples
  • Health status
    Affected
  • Subject count
    63 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
DNAs extracted from tumor and normal tissues of lung adenocarcinomas
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WGS
Target
N/A
Reagent kit
Ligation Sequencing Kit (SQK-LSK109)
Fragmentation
N/A
Platform
Oxford Nanopore Technologies PromethION
Read type
Single-end
Read length
N/A

RNA-seq

Materials and participants
lung adenocarcinoma (ICD10: C349): 6 + 5 + 26 cases (12 + 5 + 26 samples)
tumor tissues: 6 + 5 + 26 samples
normal tissues: 6 samples
  • Health status
    Affected
  • Subject count
    37 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
RNAs extracted from tumor and normal tissues of lung adenocarcinomas
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
Nextera XT DNA Library Prep Kit
SMART-Seq Stranded Kit
SMART-Seq v4 Ultra Low Input RNA Kit
TruSeq Stranded mRNA Library Prep Kit
TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold
Fragmentation
Tagmentation (Nextera XT DNA Library Preparation Kit)
Platform
Illumina HiSeq 2000
Illumina NovaSeq 6000
Read type
Paired-end
Read length
300 bp

Targeted DNA sequencing

Materials and participants
lung adenocarcinoma (ICD10: C349): 36 cases
tumor tissues: 36 samples
  • Health status
    Affected
  • Subject count
    36 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
DNAs extracted from tumor tissues of lung adenocarcinomas
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
Targeted DNA sequencing
Reagent kit
NCC Oncopanel
Fragmentation
N/A
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
300 bp

Multiplex Fluorescence Immunostaining

Materials and participants
lung adenocarcinoma (ICD10: C349): 3 + 6 cases
tumor tissues: 3 + 6 samples
  • Health status
    Affected
  • Subject count
    9 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
tumor tissues of lung adenocarcinomas
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
Multiplex fluorescence immunostaining
Sample processing
Cell treatment: Staining Kit for PhenoCycler
Platform
Akoya Biosciences PhenoCycler

Xenium 空間トランスクリプトーム

Materials and participants
lung adenocarcinoma (ICD10: C349): 1 + 1 + 10 cases
tumor tissues: 1 + 1 + 10 samples
  • Health status
    Affected
  • Subject count
    12 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
tumor tissues of lung adenocarcinomas
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
Spatial transcriptomics
Target
302 genes (Human Lung panel: 202 genes, custom: 100 genes)
Reagent kit
Xenium Decoding Consumables
Xenium Decoding Reagents
Xenium Slides & Sample Prep Reagents
Platform
10x Genomics Xenium Analyzer
Analysis method
xenium-1.1.0.2
xenium-1.5.1.2

Visium 空間トランスクリプトーム + 病理画像

Materials and participants
lung adenocarcinoma (ICD10: C349): 3 + 19 cases
tumor tissues: 3 + 25 samples
  • Health status
    Affected
  • Subject count
    22 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
RNAs extracted from tumor tissues of lung adenocarcinomas
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
Histological image
Spatial transcriptomics
Target
N/A
Reagent kit
Visium Spatial Gene Expression
Fragmentation
N/A
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
118 bp