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Dataset ID

JGAD000384

Type of data
NGS (RNA-seq, Target Capture)
Access criteria
Controlled-access (Type I)
Total data volume
682 GB
File formats
  • FASTQ
  • BAM
Research
hum0251
Date published
2022-11-29
Date modified
2022-11-29

Unrestricted-access files linked to this dataset

FileLabelSizeCopy URL
hum0251_gene_list.xlsx110 genes10.7 KB

Analysis method

RNA-seq

Materials and participants
Ph-negative B-ALL (ICD10: C91.0): 122 cases
  • Health status
    Affected
  • Subject count
    122 (Individual)
  • Age group
    Adult
Disease
Ph-negative B-ALL (C910)
Sample description
RNA extracted from leukemia cells
  • Tissue
    Leukemia cell
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
NEBNext Ultra Directional RNA Library Prep Kit for Illumina
Fragmentation
Heat treatment
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
125 bp

Targeted DNA sequencing

Materials and participants
Ph-negative B-ALL (ICD10: C91.0): 148 cases
  • Health status
    Affected
  • Subject count
    148 (Individual)
  • Age group
    Adult
Disease
Ph-negative B-ALL (C910)
Sample description
DNA extracted from leukemia cells
  • Tissue
    Leukemia cell
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
Targeted DNA sequencing
Target
110 genes
Reagent kit
SureSelect XT Low Input
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
125 bp
Reference genome
GRCh37
Mapping
bwa
Mapping quality
> 20
Coverage (depth)
570x