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Dataset ID

JGAD000367

Type of data
NGS (ATAC-seq, ChIP-seq)
Access criteria
Controlled-access (Type I)
Total data volume
132 GB
File formats
  • FASTQ
  • narrowPeak
  • bigWig
  • TXT
Research
hum0257
Date published
2021-12-24
Date modified
2023-01-04

Analysis method

ATAC-seq

Materials and participants
Pancreatic ductal adenocarcinoma (ICD10: C25.3): 17 cases
- organoids derived from tumor tissues: 20 samples
Intraductal papillary mucinous neoplasm (ICD10: D13.6): 6 cases
- organoids derived from tumor tissues: 4 samples, organoids derived from tumor-adjacent tissues: 2 samples
  • Health status
    Affected
  • Subject count
    23 (Individual)
Disease
Pancreatic ductal adenocarcinoma (C253)
Intraductal papillary mucinous neoplasm (D136)
Sample description
DNAs extracted from organoids established from pancreatic tumors
  • Tissue
    Organoid
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
ATAC-seq
Target
N/A
Reagent kit
Illumina Tagment DNA TDE1 Enzyme and Buffer Kits
Fragmentation
N/A
Platform
Illumina NextSeq 500
Read type
Paired-end
Read length
read1: 50 bp
read2: 25 bp

ChIP-seq

Materials and participants
Pancreatic ductal adenocarcinoma (ICD10: C25.3): 2 cases
[JGAD000367/E-GEAD-417] organoids derived from tumor tissues: 4 samples (2 ChIP experiments and 2 input experiments)
  • Health status
    Affected
  • Subject count
    2 (Individual)
Disease
Pancreatic ductal adenocarcinoma (C253)
Sample description
Chromatin immunoprecipitated DNAs extracted from organoids established from pancreatic tumors
  • Tissue
    Organoid
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
ChIP-seq
Target
N/A
Reagent kit
Illumina Tagment DNA TDE1 Enzyme and Buffer Kits
Fragmentation
Ultrasonic fragmentation (Bioruptor UCD-250)
Platform
Illumina NextSeq 500
Read type
Single-end
Read length
75 bp
Gene Expression Omnibus Accession
GSE202023