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Dataset ID

JGAD000366

Type of data
NGS (Exome, RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
198 GB
File formats
  • FASTQ
  • VCF
  • TXT
Research
hum0257
Date published
2021-12-27
Date modified
2022-12-28

Analysis method

WES

Materials and participants
Pancreatic ductal adenocarcinoma (ICD10: C25.3): 10 cases
- organoids derived from tumor tissues: 10 samples
Intraductal papillary mucinous neoplasm (ICD10: D13.6): 3 cases
- organoids derived from tumor tissues: 3 samples
  • Health status
    Affected
  • Subject count
    13 (Individual)
Disease
Pancreatic ductal adenocarcinoma (C253)
Intraductal papillary mucinous neoplasm (D136)
Sample description
DNAs extracted from organoids established from pancreatic tumors
  • Tissue
    Organoid
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
WES
Target
N/A
Reagent kit
SureSelect Human All Exon V6
Fragmentation
Ultrasonic fragmentation
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
100 bp
150 bp

RNA-seq

Materials and participants
Pancreatic ductal adenocarcinoma (ICD10: C25.3): 10 cases
[JGAD000366/E-GEAD-414] organoids derived from tumor tissues: 22 samples
Intraductal papillary mucinous neoplasm (ICD10: D13.6): 5 cases
[JGAD000366/E-GEAD-414] organoids derived from tumor tissues: 4 samples, organoids derived from tumor-adjacent tissues: 1 sample
  • Health status
    Affected
  • Subject count
    15 (Individual)
Disease
Pancreatic ductal adenocarcinoma (C253)
Intraductal papillary mucinous neoplasm (D136)
Sample description
RNAs extracted from organoids established from pancreatic tumors
  • Tissue
    Organoid
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
TruSeq RNA Library Prep Kit
TruSeq Stranded mRNA Library Prep Kit
Fragmentation
Heat treatment
Platform
Illumina HiSeq 2500
Illumina NovaSeq 6000
Read type
Paired-end
Read length
100 bp
150 bp