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Dataset ID

JGAD000324

Type of data
NGS (scRNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
727 GB
File formats
  • FASTQ
  • BAM
  • TXT
  • CSV
  • TAR
Research
hum0229
Date published
2020-09-28
Date modified
2025-10-29

Unrestricted-access files linked to this dataset

FileLabelSizeCopy URL
commitment_hum0229.pdf257 KB
commitment_hum0229_e.pdf140 KB

Analysis method

scRNA-seq

Materials and participants
7 supercentenarians
(7 for total RNA library, 2 of 7 for TCR library)
2 family members
2 unrelated donors
  • Health status
    Healthy
  • Subject count
    11 (Individual)
  • Age group
    Elderly
Sample description
total RNA library: RNAs extracted from PBMC
TCR library: RNAs extracted from CD4+ T Cell
  • Tissue
    CD4+ T cell, PBMC, Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
scRNA-seq
Target
TCR library: TCR region
Reagent kit
Chromium Single Cell 3' v2
Chromium Single Cell 5'
Chromium Single Cell V(D)J Enrichment Kit
Fragmentation
Enzymatic Fragmentation
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
total RNA library: 98 bp
TCR library: 150 bp