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Dataset ID

JGAD000292

Type of data
NGS (PBAT-seq, RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
218 GB
File formats
  • FASTQ
Research
hum0206
Date published
2020-09-28
Date modified
2020-11-18

Analysis method

PBAT-seq

Materials and participants
CHM (ICD10: O01): 3 cases
TS cells: 3 samples
  • Health status
    Affected
  • Subject count
    3 (Sample)
Disease
CHM (O01)
Sample description
DNAs extracted from CHM-derived TS cells
  • Tissue
    Trophoblast stem cell
Cell line
Stem cell
Sample provider
N/A
Experimental method
PBAT-seq
Target
N/A
Reagent kit
Post-Bisulfite Adaptor-Tagging (PBAT)
Fragmentation
PBAT method: fragmentation due to bisulfite conversion
Platform
Illumina HiSeq 1500
Illumina HiSeq 2500
Read type
Single-end
Read length
101 bp

RNA-seq

Materials and participants
CHM (ICD10: O01): 3 cases
CT cells: 2 samples
TS cells: 3 samples
  • Health status
    Affected
  • Subject count
    5 (Sample)
Disease
CHM (O01)
Sample description
mRNAs extracted from CT cells isolated from placenta tissues of CHMs and CHM-derived TS cells
  • Tissue
    Cytotrophoblast, Trophoblast stem cell
Cell line
Stem cell
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
TruSeq Stranded mRNA Library Prep Kit
Fragmentation
Heat treatment
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
101 bp