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Dataset ID

JGAD000263

Type of data
NGS (RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
53.9 GB
File formats
  • FASTQ
Research
hum0088
Date published
2020-09-28
Date modified
2020-11-18

Analysis method

RNA-seq

Materials and participants
2 glioblastomas (ICD10: C71.9) (TGS-01, TGS-04)
  • Health status
    Affected
  • Subject count
    2 (Individual)
Disease
glioblastomas (C719)
Sample description
mRNA extracted from a glioblastoma cell line (TGS-01) infected with Ad-EPHA6(WT), Ad-EPHA6(K757R) or Ad-LacZ (MOI 60) with or without BMP-2 stimulation for 24 h prior to RNA extraction
  • Tissue
    Glioma stem cell
  • Tumor / normal
    Tumor
Cell line
Glioma stem cell line
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
Ion Total RNA-Seq Kit v2
Fragmentation
IonProton: Enzymatic fragmentation (included in the kit: RNase III)
Platform
Thermo Fisher Scientific Ion Proton
Read type
Single-end
Read length
Ion Proton: 200 bp