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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000077

Type of data
NGS (RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
46.6 GB
File formats
  • FASTQ
Research
hum0088
Date published
2020-09-28
Date modified
2020-11-18
DDBJ Search
JGAD000077 (opens in a new tab)
JGA Study
JGAS000077 (opens in a new tab)

Analysis method

RNA-seq

Materials and participants
2 glioblastomas (ICD10: C71.9) (TGS-01, TGS-04)
  • Health status
    Affected
  • Subject count
    2 (Individual)
Disease
glioblastomas (C719)
Sample description
mRNA extracted from glioblastoma cell lines (TGS-01, TGS-04) stimulated with BMP-4 for 24 h prior to RNA extraction
  • Tumor / normal
    Tumor
Cell line
Glioma stem cell line
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
Ion Total RNA-Seq Kit v2
ScriptSeq v2 RNA-seq Library Preparation Kit
Fragmentation
Genome Analyzer IIx: Enzymatic fragmentation (included in the kit)
IonProton: Enzymatic fragmentation (included in the kit: RNase III)
Platform
Illumina Genome Analyzer IIx
Thermo Fisher Scientific Ion Proton
Read type
Mixed
Read length
Genome Analyzer IIx: 76 bp
Ion Proton: 200 bp
Data use policy
NBDC data sharing policy (JGAP000001)