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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

E-GEAD-1076

Type of data
NGS (scRNA-seq)
Access criteria
Unrestricted-access
Total data volume
309 MB
File formats
  • CSV
  • RDS
  • HDF5
Research
hum0416
Date published
2025-06-02
Date modified
2025-06-02
DDBJ Search
E-GEAD-1076 (opens in a new tab)

Analysis method

scRNA-seq

Materials and participants
Behçet's Syndrome (ICD10: M35.2): 11 cases
blood (10x chromium): 10 cases 10 samples
blood (BD Rhapsody): 4 cases 4 samples (also used in 10x chromium)
cerebrospinal fluid (CSF) (BD Rhapsody): 3 cases 3 samples (2 cases are also used in 10x chromium)
  • Health status
    Affected
  • Subject count
    11 (Individual)
Disease
Behçet's Syndrome (M352)
Sample description
RNAs extracted from a single cell isolated from blood and CSF samples
  • Tissue
    Cerebrospinal fluid, Peripheral blood
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 5' v2
BD Rhapsody WTA Reagent Kit
Fragmentation
Enzymatic Fragmentation
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
10x chromium: 91 bp
BD Rhapsody: 150 bp
Reference genome
GRCh38
QC and filtering
10x chromium: Cell Ranger Matrics
BD Rhapsody: BD Rhapsody Sequence Analysis Pipeline basic algorithm for putative cell identification
Analysis method
10x chromium: CellRanger (version 6.0.6)
BD Rhapsody: BD Rhapsody Sequence Analysis Pipeline (version 1.12)
Processed data type
Gene expression matrix
Data use policy
NBDC data sharing policy (JGAP000001)