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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

DRA019274

Type of data
NGS (CITE-seq, BD Ab-seq)
Access criteria
Unrestricted-access
Total data volume
1.7 TB
File formats
  • FASTQ
Research
hum0416
Date published
2024-09-18
Date modified
2024-09-18
DDBJ Search
DRA019274 (opens in a new tab)

Unrestricted-access files linked to this dataset

Download the URL list
FileLabelSizeCopy URL
Abseq_list.xlsxInformation on the antibodies used for antibody sequencing9.7 KB

Analysis method

CITE-seq

Materials and participants
systemic sclerosis (ICD10: M34): 21 cases
blood samples were collected from a patient three times and 20 patients once
6 healthy donors (3 are also used in E-GEAD-635)
  • Health status
    Mixed
  • Subject count
    27 (Individual)
Disease
systemic sclerosis (M34)
Sample description
RNAs extracted from a single cell isolated from PBMCs
  • Tissue
    PBMC, Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
CITE-seq
Target
N/A
Fragmentation
Enzymatic fragmentation
Platform
Illumina HiSeq 2500
Illumina NovaSeq 6000
Read type
Paired-end
Read length
91 bp
Reference genome
GRCh38
QC and filtering
Cell Ranger Metrics
Analysis method
CellRanger (version 6.0.6)
Data use policy
NBDC data sharing policy (JGAP000001)

CITE-seq (BD Ab-seq)

Materials and participants
SSc (ICD10: M34) with renal crisis: 1 case
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
SSc (M34)
Sample description
RNAs extracted from a single cell isolated from whole blood leukocytes and kidney biopsy specimens
  • Tissue
    Kidney, Leukocyte, Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
CITE-seq
Target
Information on the antibodies used for antibody sequencing
Reagent kit
BD Rhapsody WTA Reagent Kit
Fragmentation
Enzymatic Fragmentation
Platform
Illumina HiSeq 2500
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Reference genome
GRCh38
QC and filtering
Cell Ranger Metrics
Analysis method
BD Rhapsody Sequence Analysis Pipeline (version 1.12)
Data use policy
NBDC data sharing policy (JGAP000001)