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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Research ID

hum0490-v1Release info

Latest

Research title

An investigation into the effects of progestin on follicular development and oocyte maturation during controlled ovarian stimulation using the Progestin-Primed Ovarian Stimulation (PPOS)

Research overview

Aims
In recent years, the Progestin-Primed Ovarian Stimulation (PPOS) method, utilizing progestin as ovulation suppressants, has gained traction as a controlled ovarian stimulation strategy in assisted reproductive technology. However, the impact of progestin agents on follicular fluid, mural granulosa cell (mGC), and cumulus cells at the levels of gene and protein expression has not been fully assessed. This study aims to evaluate the effects of progestin agents used in the PPOS method, on follicular development, and oocyte maturation.
Methods
Collected and washed mGCs were cryopreserved in Bambanker medium at −80 °C. After thawing, cell counts were standardised across patients and normalised to those of the patient with the minimum cell count. Cells were mixed within each group to form the PPOS and GnRH-ant groups. Dead cells were removed through magnetic cell sorting (Dead Cell Removal Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) with LS columns and pre-separation filters from the MidiMACS™ Starting Kit (Miltenyi Biotec), following DNase treatment. After library preparation using Chromium Next GEM Single Cell V(D)J Reagent Kits, single cell RNA-seq was performed using a DNBSEQ-G400 sequencer.
Participants/materials
mGCs from 16 infertility patients with normal ovarian function. Eight patients were treated with conventional ovulation induction (GnRH-ant) and eight patients were treated with PPOS.
URL
N/A

Datasets

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD000911NGS (scRNA-seq)
  • scRNA-seq
Controlled-access (Type I)2024-12-20

Data provider

Principal investigator
Mika Handa
Affiliation
Obstetrics and Gynecology, Osaka University

Research projects

No research projects.

Grants

NameTitleProject number
KAKENHI Grant-in-Aid for Scientific Research (C)
Elucidating the Mechanism of Progestin Effects on Oocytes and Establishing an Optimal Treatment Protocol for the PPOS Method
  • 23K08867

Related publications

TitleDOIDataset ID
Adverse effects of progestin-primed ovarian stimulation: Combination of clinical study and single cell analysis
https://doi.org/10.1016/j.rbmo.2025.104833 (opens in a new tab)

Controlled access users

No use of the controlled access data has been recorded.