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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Research ID

hum0477-v2Release info

Latest

Research title

Tumor tissue culture and its characterization in gastrointestinal cancer

Research overview

Aims
Cancer is the leading cause of death in Japan and is continuing to rise. It has been reported that tumor microenvironment is linked to the tumor development, invasion, metastasis and residence of cancer to therapy. Organoid can reproduce its organ structure and function, there are expectations for their application in biomedical research, regenerative medicine, and human disease study. We collected tissues from patients with gastrointestinal tract or suspected tumors treated at Gunma University Hospital to avoid inconveniences in diagnosis and treatment. We perform organoid culture, analyzing their properties and mechanism related to cancer cells and microenvironment. This research is expected to identify mechanisms of tumor development, novel therapeutic targets and develop new therapies.
Methods
RNA-seq analysis of epithelial cells from human colon organoid and Caco-2 cells was conducted before and after co-cultivation of epithelial cells and Bifidobacterium longum subsp. longum (B. longum) for 24 hours.
Participants/materials
Healthy tissues from a colorectal cancer patient (before co-cultivation with B. longum 3 sample, after co-cultivation with B. longum 3 + 3 samples and cultivation without B. longum 7 sample)
Caco-2 cells (before co-cultivation with B. longum 3 sample, after co-cultivation with B. longum 3 + 3 samples and cultivation without B. longum 7 sample)
URL
N/A

Datasets

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD000875NGS (RNA-seq)
  • RNA-seq
Controlled-access (Type I)2024-10-18

Data provider

Principal investigator
Nobuo Sasaki
Affiliation
Laboratory of Mucosal Ecosystem Design, Institute for Molecular and Cellular Regulation, Gunma University

Research projects

No research projects.

Grants

NameTitleProject number
Project Focused on Developing Key Technology for Discovering and Manufacturing Drugs for Next-Generation Treatment and Diagnosis, Japan Agency for Medical Research and Developmstent (AMED)
Development of Scalable Platform of the Drug Discovery Targeting for Human Mucosal Ecosystem
  • JP23ae0121046
Fusion Oriented REsearch for disruptive Science and Technology (FOREST) program, Japan Science and Technology Agency (JST)
Development of a novel culturing system for gut bacteria to regulate adult tissue stem cells
  • JPMJFR2161
KAKENHI Grant-in-Aid for Scientific Research (B)
Generating the next generation of organoid biobank to reveal intestinal environment-dependent tumorigenesis
  • 23H02713
KAKENHI Grant-in-Aid for Challenging Research (Pioneering)
Predictive modeling of bacteria variation using in vitro reconstructions of the human gut ecosystem
  • 23K17415

Related publications

No related publications.

Controlled access users

No use of the controlled access data has been recorded.