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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Research ID

hum0472-v1Release info

Latest

Research title

Non-clinical research for the development of neuronal cells from human iPS cell derived cerebral cortical organoid

Research overview

Aims
When cerebral cortical neurons are damaged due to stroke or trauma, the injured neurons typically do not recover, resulting in persistent sequelae such as motor paralysis. One potential therapeutic approach is the transplantation of neurons derived from pluripotent stem cells. While we have demonstrated that cerebral organoids can be differentiated from human ES cells, using human iPS cells is more desirable for clinical applications due to the possibility of allogeneic transplantation with HLA compatibility. Here, we will perform non-clinical trials to establish differentiation protocols from hiPSCs to cerebral cortical neurons and assess the safety and efficacy of these differentiated cells. Additionally, we will explore large-scale cell production for future industrialization.
Methods
Organoids were dissociated into single cells with Neuron Dissociation Solutions. Dissociated cells were re-suspended with HBSS supplemented with 10% (v/v) KSR and 10μM Y-27632 at 1,000 cells/µL density. Cell suspension was loaded onto a Chromium Next GEM Chip G (2000177 10X Genomics), targeting 3,000 cells for capture per well, and processed in the Chromium controller to obtain Gel Beads-in-Emulsion. Libraries were generated and RNA-seq was performed.
Participants/materials
Organoids derived from induced pluripotent stem cells of a single healthy individual.
URL
N/A

Datasets

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD000859NGS (scRNA-seq)
  • scRNA-seq
Controlled-access (Type I)2024-08-27

Data provider

Principal investigator
Jun Takahashi
Affiliation
Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University

Research projects

No research projects.

Grants

NameTitleProject number
Acceleration Program of R&D and Implementation for Regenerative Medicine and Cell and Gene Therapy, Japan Agency for Medical Research and Development (AMED)
Translational study for iPS cell-based therapy for stroke
  • JP23bm1223005
Japan Agency for Medical Research and Development (AMED)
Study of iPS cell-based therapy for stroke
  • JP24ym0126160

Related publications

TitleDOIDataset ID
Validation of non-invasive morphology-based selection of cerebral cortical organoids by paired morphological and single-cell RNA sequencing analyses
https://doi.org/10.1016/j.stemcr.2024.09.005 (opens in a new tab)

Controlled access users

No use of the controlled access data has been recorded.