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Research ID

hum0464-v1Release info

This page is a past version (v1). The latest version is v2.
Latest version (v2)

Research title

In Vitro Amplification of Human Hematopoietic Tumor Cells and Construction of Mouse Models for Reproduction of Pathological Conditions

Research overview

Aims
Radical treatments for hematopoietic tumors such as leukemia, myelodysplastic syndromes, myeloproliferative tumors, and multiple myeloma have not been fully established. One of the major factors hindering the efforts to find such treatments is the difficulty in constructing model mice that appropriately mimic the disease state. In this study, we collected peripheral blood or bone marrow cells from patients with hematological tumors and attempted in vitro cultures and molecular cell biological analyses, including genetic and epigenetic studies. Based on the knowledge gained from these studies, we aim to create patient-derived xenograft mouse models of hematological tumors and further develop new therapeutic approaches.
Methods
A part of bone marrow aspirates obtained to establish a definite diagnosis or evaluate the disease status was provided for this study. Mononuclear cells were separated by gradient separation using Ficoll, and then subjected to magnetic beads separation or FACS sorting to purify tumor cells. Next, they were subjected to RNA sequencing to analyze gene expression profiles.
Participants/materials
[RNA-seq] A total of 86 + 14 bone marrow samples from 17 multiple myelomas, 3 normal bone marrow samples
[single-cell RNA-seq, scBCR-seq] A total of 22 bone marrow samples from 9 multiple myelomas
URL
N/A

Datasets

The list is the one this version published; each dataset's content is shown as it is now.

CartDataset IDType of dataAnalysis methodAccess criteriaDate published
JGAD000856NGS (RNA-seq, scRNA-seq, scBCR-seq)
  • RNA-seq
  • scRNA-seq + scBCR-seq
Controlled-access (Type I)2024-07-25

Data provider

Principal investigator
Atsushi Iwama
Affiliation
Division of Stem Cell and Molecular Medicine Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo

Research projects

No research projects.

Grants

NameTitleProject number
KAKENHI Grant-in-Aid for Scientific Research (S)
Pathogenec implication of hematopoietic stem cell aging in age-associated hematological malignancies
  • JP24H00066

Related publications

No related publications.

Controlled access users

No use of the controlled access data has been recorded.