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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000856

Type of data
NGS (RNA-seq, scRNA-seq, scBCR-seq)
Access criteria
Controlled-access (Type I)
Total data volume
288 GB
File formats
  • FASTQ
Research
hum0464
Date published
2024-07-25
Date modified
2025-06-19
DDBJ Search
JGAD000856 (opens in a new tab)
JGA Study
JGAS000723 (opens in a new tab)

Analysis method

RNA-seq

Materials and participants
multiple myeloma (ICD10: C90.0): 17 cases
bone marrow: 86 + 14 samples
11 fractions: 8 cases (60 + 2 samples)
CD138 positive/negative cell pairs: 13 cases (26 + 12 samples)
normal bone marrow: 3 samples
plasma cell fraction: 3 samples
  • Health status
    Mixed
  • Subject count
    244 (Mixed)
Disease
multiple myeloma (C900)
Sample description
RNAs extracted from 11 fractions of cells, CD138+/- cells or plasma cell fractions of normal bone marrow samples
  • Tissue
    Bone marrow
  • Tumor / normal
    Mixed
Sample provider
normal bone marrow samples (Lonza, ＃2M-125C)
Experimental method
RNA-seq
Target
N/A
Reagent kit
NEBNext Ultra DNA Library Prep Kit for Illumina
SMART-Seq HT Kit
SMART-Seq v4 Ultra Low Input RNA Kit
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
Illumina HiSeq 2000
Illumina NextSeq 2000
Illumina NextSeq 500
Read type
Mixed
Read length
70 bp
90 bp
Data use policy
NBDC data sharing policy (JGAP000001)

scRNA-seq + scBCR-seq

Materials and participants
multiple myeloma (ICD10: C90.0): 9 cases
bone marrow: 22 samples
(scRNA-seq: 11 samples, scBCR-seq: 11 samples)
  • Health status
    Affected
  • Subject count
    31 (Mixed)
Disease
multiple myeloma (C900)
Sample description
RNAs extracted from CD38+ cells
  • Tissue
    Bone marrow
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
BCR-seq
scRNA-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 5' v2
Chromium Single Cell Human BCR Amplification Kit
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
Illumina HiSeq 2000
Read type
Paired-end
Read length
90 bp
Data use policy
NBDC data sharing policy (JGAP000001)