Research ID
hum0443-v1Release info
Latest
Research title
Relationship between changes in the T-cell receptor repertoire of regulatory T cells before and after pregnancy and pregnancy outcome.
Research overview
- Aims
- A fetus expresses paternally derived antigens which is allogenic for a mother, however; fetus is not rejected from maternal immune cells because of feto-maternal immune tolerance during pregnancy. At the same time, maternal immune system still maintains their ability to respond against pathogenic organisms. The adequate balance between tolerogenic regulatory T cells (Treg) and proinflammatory CD4+ T cells and cytotoxic T cells at feto-maternal interface is important for healthy pregnancies. However, diversity of CD4+ T cells and Tregs in healthy pregnancies and its alternation in preeclampsia (PE) have not been fully understood. Therefore, we conducted single-cell mRNA sequencing and T cell receptor repertoire analysis to reveal a landscape of diverse CD4 T cells and Treg in healthy pregnancies and altered gene expression patterns which relate to pathogenesis of PE.
- Methods
- Lymphocytes were isolated from decidual tissue and peripheral blood. CD4+ T cells were sorted and subjected into BD Rhapsody Single-Cell Analysis System. Targeted RNA-sequence and TCR sequence libraries were constructed. Sequencing was performed using Illumina NovaSeq 6000.
- Participants/materials
- Healthy early pregnancy decidual CD4+ T cells (n=4), Healthy late pregnancy decidual CD4+ T cells (n=3), Preeclampsia decidual CD4+ T cells (n=3), Healthy late pregnancy peripheral blood CD4+ T cells (n=1) . One pairs of healthy late decidua and peripheral blood was from the same subject. All samples were subjected to targeted RNA-sequencing with the BD Rhapsody. TCR-sequence was performed in 2 healthy early, 1 healthy late decidua, 1 healthy late peripheral blood, and 1 preeclampsia decidua.
- URL
- N/A
Datasets
| Cart | Dataset ID | Type of data | Analysis method | Access criteria | Date published |
|---|---|---|---|---|---|
| DRA017833 | NGS (Target RNA-seq, TCR-seq) |
| Unrestricted-access | 2024-04-26 | |
| E-GEAD-674 | NGS (Target RNA-seq, TCR-seq) |
| Unrestricted-access | 2024-04-26 |
Data provider
- Principal investigator
- Akitoshi Nakashima
- Affiliation
- Department of Obstetrics and Gynecology, University of Toyama
Research projects
No research projects.
Grants
| Name | Title | Project number |
|---|---|---|
KAKENHI Grant-in-Aid for Scientific Research (C) | The crosstalk of paternal antigen specific Treg cells and dendritic cells in feto-maternal tolerance |
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KAKENHI Grant-in-Aid for Early-Career Scientists | Immunological differece between pregnancy and cancer in terms of T cell receptor repertoire. |
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KAKENHI Grant-in-Aid for Early-Career Scientists | Single T cell transcriptome analysis for biomarker discovery in the treatment of preeclampsia. |
|
KAKENHI Fund for the Promotion of Joint International Research (Fostering Joint International Research (A)) | Elucidation of pathogenesis mechanisms of preeclampsia focusing on cell-cell interaction between maternal CD4 positive T cells and trophoblasts. |
|
KAKENHI Grant-in-Aid for Scientific Research (C) | New mechanism of autophagy inhibition from the study of preeclampsia |
|
KAKENHI Grant-in-Aid for Scientific Research (B) | Failure of autophagy for the therapeutic target of preeclampsia |
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Project for Baby and Infant Research of Health and Development to Adolescent and Young adult, Japan Agency for Medical Research and Development (AMED) | Research on elucidation of causes and preventive treatment of recurrent pregnancy loss |
|
Related publications
| Title | DOI | Dataset ID |
|---|---|---|
CD4+ T cell heterogeneity in gestational age and preeclampsia using single-cell RNA sequencing |