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Dataset ID

E-GEAD-674

Type of data
NGS (Target RNA-seq, TCR-seq)
Access criteria
Unrestricted-access
Total data volume
3.1 MB
File formats
  • TXT
  • CSV
Research
hum0443
Date published
2024-04-26
Date modified
2024-04-26
DDBJ Search
E-GEAD-674 (opens in a new tab)

Analysis method

Target RNA-seq

Materials and participants
Healthy pregnancy: 7 subjects (early pregnancy: 4 subjects, late pregnancy: 3 subjects)
Preeclampsia (ICD10: O149): 3 cases
Healthy early pregnancy decidual CD4+ T cells: 4 samples
Healthy late pregnancy decidual CD4+ T cells: 3 samples
Preeclampsia decidual CD4+ T cells: 3 samples
Healthy late pregnancy peripheral blood CD4+ T cells: 1 sample
  • Health status
    Mixed
  • Subject count
    10 (Mixed)
  • Sex
    Female
Disease
Preeclampsia (O149)
Sample description
RNAs extracted from CD4+ T cells isolated from decidual tissue and peripheral blood
  • Tissue
    Decidua, Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
RNA-seq
Target
1) BD Rhapsody T Cell Expression Panel Hs (BD Biosciences Cat#633751)
2) Rhapsody Supplemental Gene Primer for IL10 and NRP1.
3) T cell receptor
Reagent kit
BD Rhapsody Targeted mRNA and AbSeq Kit
Fragmentation
N/A
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
531 bp (Mean)
Data use policy
NBDC data sharing policy (JGAP000001)

TCR-seq

Materials and participants
Healthy pregnancy: 3 subjects (early pregnancy: 2 subjects, late pregnancy: 1 subject)
Preeclampsia (ICD10: O149): 1 case
Healthy early pregnancy decidua: 2 samples
Healthy late pregnancy decidua: 1 sample
Preeclampsia decidua: 1 sample
Healthy late pregnancy peripheral blood: 1 sample
  • Health status
    Mixed
  • Subject count
    4 (Individual)
  • Sex
    Female
Disease
Preeclampsia (O149)
Sample description
RNAs extracted from CD4+ T cells isolated from decidual tissue and peripheral blood
  • Tissue
    Decidua, Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
TCR-seq
Target
N/A
Reagent kit
BD Rhapsody Targeted mRNA and AbSeq Kit
Fragmentation
N/A
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
TCRα: 659 bp (Mean)
TCRβ: 655 bp (Mean)
Data use policy
NBDC data sharing policy (JGAP000001)