Research ID
hum0431-v1Release info
Latest
Research title
Genetic analysis aimed at elucidating the pathophysiology, vulnerability to onset, treatment responsiveness of neuropsychiatric disorders, and developing new treatments and diagnostic and preventive methods
Research overview
- Aims
- Identification of susceptibility gene clusters for neuropsychiatric disorders such as schizophrenia, mood disorders (bipolar disorder, major depressive disorder), developmental disorders, and epilepsy.
- Methods
- RNA-seq analysis
- Participants/materials
- We introduced single variants identified in patients with Autism Spectrum Disorder (ASD) into human iPSCs (HPS4290:201B7-Ff]) using the CRISPR/Cas9 system. Variants at the protospacer adjacent motif (PAM) sequence were also introduced.
1) Mutant iPSC lines with the chr7:g.26200781C>T variant and the single PAM sequence: 2 samples
Control lines with a single PAM sequence: 2 samples
2) Mutant iPSC lines with the chr22:g.19179942C>T variant and the single PAM sequence: 3 samples
Control lines with a single PAM sequence: 2 samples - URL
- N/A
Datasets
| Cart | Dataset ID | Type of data | Analysis method | Access criteria | Date published |
|---|---|---|---|---|---|
| JGAD000781 | NGS (RNA-seq) |
| Controlled-access (Type I) | 2024-01-25 |
Data provider
- Principal investigator
- Atsushi Takata
- Affiliation
- Laboratory for Molecular Pathology of Psychiatric Disorders, RIKEN Center for Brain Science
Research projects
No research projects.
Grants
| Name | Title | Project number |
|---|---|---|
Strategic Research Program for Brain Sciences, Japan Agency for Medical Research and Development (AMED) | Identification of genetically defined subgroups of bipolar disorder by sequence analysis of trio samples |
|
Advanced Genome Research and Bioinformatics Study to Facilitate Medical Innovation (GRIFIN), Platform Program for Promotion of Genome Medicine, Japan Agency for Medical Research and Development (AMED) | Analysis of the genetic architectures of human diseases based on the oligogenic model |
|
The Strategic International Brain Science Research Promotion Program (Brain/MINDS Beyond), Japan Agency for Medical Research and Development (AMED) | Analysis of somatic mutations in bipolar disorder and methodological development of genomic neuropathology |
|
KAKENHI Grant-in-Aid for Transformative Research Areas (B) | Identification of genes and key molecular pathways determining cluster/hub cells and analysis of their relationship to human diseases |
|
KAKENHI Grant-in-Aid for Scientific Research (B) | Comprehensive study of rare germline and somatic mutations by large-scale sequence analysis for bipolar disorder |
|
KAKENHI Grant-in-Aid for Early-Career Scientists | Molecular pathological analysis of a histone methyltransferase associated with autism spectrum disorder |
|
KAKENHI Grant-in-Aid for Research Activity Start-up | Identification of loci associated with paternal age effect of Denovo mutation by large-scale family genome data analysis |
|
Related publications
| Title | DOI | Dataset ID |
|---|---|---|
Topologically associating domains define the impact of de novo promoter variants on autism spectrum disorder risk |
Controlled access users
No use of the controlled access data has been recorded.