hum0302-v1Release info

iPS Cell Advanced Characterization and Development of modified iPS cells

Aims

To characterize iPS cell lines (healthy-donor iPS cell lines and disease-specific iPS cell lines), differentiation potential analysis (evaluation of differentiation potential into disease-causing cells and involved cells), disease-causing gene analysis, and whole genome analysis were performed.

Methods

[JGAS000382] Hepatocytes were induced to differentiate from human iPS cells and collected on day 19. RNA was collected and strand-specific library preparation was performed by a PolyA selection method. The prepared library was sequenced by Novaseq6000. Sequencing was performed in a 2x150 bp PE configuration with a data output of about 6 Gb per sample (equivalent to about 20 million paired reads).
[JGAS000683] Kidney organoids were induced to differentiate from human iPS cells and collected on day 18. RNA was collected and strand-specific library preparation was performed by a PolyA selection method. The prepared library was sequenced by Novaseq6000. Sequencing was performed in a 2x150 bp PE configuration with a data output of about 6 Gb per sample (equivalent to about 20 million paired reads).

Participants/materials

iPS cells derived from patients with Wilson's disease, juvenile nephron tabes (NPH1) patients and iPS cells derived from healthy donors

URL

• https://acd.brc.riken.jp/en/ (opens in a new tab)

Principal investigator

Yohei Hayashi

Affiliation

RIKEN BioResource Research Center

No grants.

No use of the controlled access data has been recorded.