Aims: To explore genetic alterations in solid tumors, we performed genome wide analysis using next generation sequencing methods.

Methods: JGAS000036: Whole exome sequencing (WES) for 16 rhabdomyosarocoma (RMS) and 7 pleuropulmonary blastoma (PPB) patients using tumor tissues and corresponding normal blood cells (non-tumor tissues) were performed. Relapsed tumor tissues were also analyzed in total of 5 cases. JGAS000046: SNP-chip analysis and targeted amplicon deep sequencing were performed using tumor tissues and corresponding normal blood cells (non-tumor tissues) from 522 neuroblastoma (NB) patients and 39 neuroblastoma cell lines. JGAS000088: SNP-chip and WES analyses were performed using tumor tissues and corresponding normal blood cells (non-tumor tissues) from 10 pancreatoblastoma (PBL) patients. Methylation array and RNA-seq analyses were performed using only tumor tissues. JGAS000188: SNP-chip, Methylation array, RNA-seq, and Target Capture Sequencing analyses were performed using tumor tissues from 59 hepatoblastoma (HBL) patients and 2 HBL cell lines. JGAS000204: SNP-chip, Methylation array, RNA-seq, and Target Capture Sequencing analyses were performed using tumor tissues from 51 patients with pediatric germ cell tumors. JGAS000246: RNA-seq and Target Capture Sequencing analyses were performed using tumor tissues from 30 high-risk neuroblastoma patients.

Targets: JGAS000036: 16 RMSs and 7 PPBs JGAS000046: 522 NBs and 39 neuroblastoma cell lines JGAS000088: 10 PBLs JGAS000188: 59 HBLs and 2 HBL cell lines JGAS000204: 51 patients with pediatric germ cell tumors JGAS000246: 30 high-risk neuroblastoma patients