Research ID
hum0035-v1Release info
This page is a past version (v1). The latest version is v5.
Latest version (v5)Research title
Genome wide analysis of gene mutations in solid tumors
Research overview
- Aims
- To explore genetic alterations in solid tumors, we performed genome wide analysis using next generation sequencing methods.
- Methods
- JGAS000036: Whole exome sequencing (WES) for 16 rhabdomyosarocoma (RMS) and 7 pleuropulmonary blastoma (PPB) patients using tumor tissues and corresponding normal blood cells (non-tumor tissues) were performed. Relapsed tumor tissues were also analyzed in total of 5 cases.
JGAS000046: SNP-chip analysis and targeted amplicon deep sequencing were performed using tumor tissues and corresponding normal blood cells (non-tumor tissues) from 522 neuroblastoma (NB) patients and 39 neuroblastoma cell lines.
JGAS000088: SNP-chip and WES analyses were performed using tumor tissues and corresponding normal blood cells (non-tumor tissues) from 10 pancreatoblastoma (PBL) patients. Methylation array and RNA-seq analyses were performed using only tumor tissues.
JGAS000188: SNP-chip, Methylation array, RNA-seq, and Target Capture Sequencing analyses were performed using tumor tissues from 59 hepatoblastoma (HBL) patients and 2 HBL cell lines.
JGAS000204: SNP-chip, Methylation array, RNA-seq, and Target Capture Sequencing analyses were performed using tumor tissues from 51 patients with pediatric germ cell tumors.
JGAS000246: RNA-seq and Target Capture Sequencing analyses were performed using tumor tissues from 30 high-risk neuroblastoma patients. - Participants/materials
- JGAS000036: 16 RMSs and 7 PPBs
JGAS000046: 522 NBs and 39 neuroblastoma cell lines
JGAS000088: 10 PBLs
JGAS000188: 59 HBLs and 2 HBL cell lines
JGAS000204: 51 patients with pediatric germ cell tumors
JGAS000246: 30 high-risk neuroblastoma patients - URL
- N/A
Datasets
The list is the one this version published; each dataset's content is shown as it is now.
| Cart | Dataset ID | Type of data | Analysis method | Access criteria | Date published |
|---|---|---|---|---|---|
| JGAD000036 | NGS (Exome) for RMS and PPB |
| Controlled-access (Type I) | 2020-09-28 |
Data provider
- Principal investigator
- Junko Takita
- Affiliation
- Graduate School of Medicine, The University of Tokyo
Research projects
| Name | URL |
|---|---|
Department of Pediatrics, Hematology/Oncology Group | N/A |
Project for Development of Innovative Research on Cancer Therapeutics -Investigation of novel molecular targets of intractable pediatric solid tumors using integrated genetic analysis- | N/A |
Grants
| Name | Title | Project number |
|---|---|---|
Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT), Japan Agency for Medical Research and Development (AMED) | Investigation of novel molecular targets of intractable pediatric solid tumors using integrated genetic analysis |
|
KAKENHI Grant-in-Aid for Scientific Research (B) | Studies on clonal evolutions which regulate metastasis and relapse in pediatric solid tumors using spatiotemporal and comprehensive genome-wide analysis |
|
Children's Cancer Association of Japan: Therapeutic Research Grants | Comprehensive genetic analysis and searching for drug target discovery in pediatric germ cell tumors | N/A |
Related publications
| Title | DOI | Dataset ID |
|---|---|---|
Integrated genetic and epigenetic analysis defines novel molecular subgroups in rhabdomyosarcoma. | ||
Biallelic DICER1 mutations in sporadic pleuropulmonary blastoma. | ||
Identification of the genetic and clinical characteristics of neuroblastomas using genome-wide analysis. | ||
Integrated molecular characterization of the lethal pediatric cancer pancreatoblastoma. | ||
Integrated multiomics analysis of hepatoblastoma unravels its heterogeneity and provides novel druggable targets | ||
Comprehensive genetic analysis of pediatric germ cell tumors identifies potential drug targets |
Controlled access users
| Principal investigator | Affiliation | Country/Region | Research title | Period of data use | Dataset ID |
|---|---|---|---|---|---|
| Jiguang Wang | WangLab, The Hong Kong university of science and technology | China | Germline and somatic mutations in pediatric germ cell tumor. | 2022-06-03 – 2024-04-03 | |
| Weng Chuan Peng | Research, Princess Maxima Center for Pediatric Oncology | Netherlands | Towards understanding pediatric liver tumors | 2023-08-21 – 2027-06-01 | |
| Michiaki Hamada | Hamada Laboratory, Faculty of Science and Engineering, Waseda University | Japan | Construction of RNA-targeted Drug Discovery Database | 2023-01-05 – 2027-10-31 | |
| Yujie Tang | Department of Histoembryology, Genetics and Developmental Biology, College of Basic Medical Sciences, Shanghai Jiao Tong University | China | The key molecular mechanisms underlying the oncogenesis and progression of neuroblastoma and hepatoblastoma | 2023-03-08 – 2027-12-31 | |
| leendert looijenga | Research and Development, Looijenga Group, Prinses Maxima Centrum | Netherlands | Histological and molecular characterization of extracranial germ cell tumors: towards an optimal patient risk stratification. | 2025-04-28 – 2027-03-31 | |
| Ching Lau | Pediatric Oncology, The Jackson Lab for Genomic Medicine | Connecticut, United States | Development of new DNA methylation-based classifier for brain tumors | 2026-04-06 – 2027-01-16 |