HumanDBs

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NBDC Research ID:hum0009-v3

Release info

Latest

Research title

DNA Methylation Analysis of Early Human Embryos

Research overview

Aims: To generate single-base resolution DNA methylome maps of human gametes and embryos and uncover DNA methylation dynamics during early human development.

Methods: Whole genome bisulfite sequencing libraries were prepared using the post-bisulfite adaptor tagging (PBAT) method. PBAT libraries were sequenced on the HiSeq 2000 or 2500 platform (Illumina) with 100 bp single-end reads.

Targets: Human oocytes, sperm, preimplantation embryos, and cord blood cells

Datasets

Dataset ID	Access type	Type of data	Release date
DRA003802	Unrestricted-access	NGS (PBAT-seq)	2015-07-31
JGAD000006	Controlled-access (Type I)	NGS (PBAT-seq)	2020-09-28

Data provider

Principal investigator: Takahiro Arima
Name: Department of Informative Genetics, Environment and Genome Research Center,Tohoku University Graduate School of Medicine

Research projects

Name	URL
No data

Grants

Name	Title	Project number
Core Research and Evolutional Science and Technology, Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED-CREST)	Epigenome analysis of cells in the placenta and endometrium forming the fetal-maternal interface	JP17gm0510011
KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas	Epigenome Dynamics and Regulation in Germ Cells	26112502

Related publications

Title	DOI	Dataset ID
Genome-Wide Analysis of DNA Methylation Dynamics during Early Human Development	https://doi.org/10.1371/journal.pgen.1004868	DRA003802 JGAD000006

Controlled access users

Principal Investigator	Affiliation	Country/Region	Research title	Period of data use	Dataset ID
Skaar David	North Carolina State University	USA	Epigenetics and Genetics Throughout the Lifespan	2019-04-15—2021-02-02	JGAD000006
Monk David	Bellvitge Institute for Biomedical Research (IDIBELL)	Spain	To trace the germline origin of methylated regions in the placenta. To identify oocyte-derived methylation that is aberrantly lost during human parthenogenetic stem cell generation.	2015-04-07—2015-08-04	JGAD000006
Toh Hidehiro	Medical Institute of Bioregulation Kyushu University	Japan	Epigenome dynamics and regulation in germ cells	2015-04-01—2015-08-04	JGAD000006