NBDC Research ID: hum0544.v1
SUMMARY
Aims: The testis is known to be the tissue with the fastest evolutionary rate, and its properties vary greatly between species. Spermatogonial stem cells (SSCs) are the stem cells responsible for spermatogenesis in the adult testis. Previous research using monkeys has revealed that the SSC system in primates differs significantly from that in mice. This study aims to understand the primate SSC system and investigate the causes of human male infertility.
Methods: Testes were dispersed with collagenase 4 and trypsin. SSEA4-positive cells were collected by FACS and used for single-cell bisulfite-seq analysis.
Participants/Materials: Normal testes from obstructive azoospermia
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000888 | NGS (scWGBS) | Controlled-access (Type I) | 2026/03/10 |
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MOLECULAR DATA
| Participants/Materials |
obstructive azoospermia (ICD10: N46): 1 case normal testes: 1 sample |
| Targets |
scWGBS Libraries were prepared by using the protocol reported by Zhou et al. with minor modifications. |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq X] |
| Library Source | DNAs extracted from SSEA4-positive cells isolated from normal testes |
| Cell Lines | - |
| Library Construction (kit name) | NEBNext Multiplex Oligos for Illumina, 96 Unique Dual Index kit |
| Fragmentation Methods | - |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100-500 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD0001032 |
| Total Data Volume | 75.4 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Toshiaki Watanabe
Affiliation: Center for Regenerative Medicine, National Center for Child Health and Development
Project / Group Name: Primate/human germ cell research project/Watanabe group
URL: https://www.amed.go.jp/content/000153312.pdf
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Precursory Research for Innovative Medical care (PRIME), Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED) | Study of the establishment of DNA methylation during primate germ cell development | JP22gm6310010 |
PUBLICATIONS
USRES (Controlled-access Data)
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Establishment of DNA methylation during primate germ cell development |
JGAD001031 JGAD001032 |
|
| 2 |
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
