NBDC Research ID: hum0542.v1
SUMMARY
Aims: Previous studies using monkeys have revealed the timing and mechanism of DNA methylation establishment in primate germ cells. The purpose of this study is to understand the establishment of DNA methylation in human testicular and ovarian germ cells. This study will advance our understanding of human germ cell development and contribute to the prevention of abnormal gamete DNA methylation and the development of infertility treatments.
Methods: single-cell whole genome bisulfite-sequencing
Participants/Materials: Three testicular cancer patients
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000887 | NGS (scWGBS) | Controlled-access (Type I) | 2026/03/09 |
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MOLECULAR DATA
| Participants/Materials |
testicular teratoma (ICD10: C629): 3 cases non-tumor tissues: 3 samples (normal testes in teratomas) |
| Targets |
scWGBS Libraries were prepared by using the protocol reported by Zhou et al. with minor modifications. |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq X] |
| Library Source | DNAs extracted from germ cells isolated from normal testes |
| Cell Lines | - |
| Library Construction (kit name) | NEBNext Multiplex Oligos for Illumina, 96 Unique Dual Index kit |
| Fragmentation Methods | - |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100-500 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD0001031 |
| Total Data Volume | 338.9 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Toshiaki Watanabe
Affiliation: Center for Regenerative Medicine, National Center for Child Health and Development
Project / Group Name: Primate/human germ cell research project/Watanabe group
URL: https://www.amed.go.jp/content/000153312.pdf
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Precursory Research for Innovative Medical care (PRIME), Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED) | Study of the establishment of DNA methylation during primate germ cell development | JP22gm6310010 |
PUBLICATIONS
USRES (Controlled-access Data)
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Establishment of DNA methylation during primate germ cell development |
JGAD001031 JGAD001032 |
|
| 2 |
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
