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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

NHA000168

Type of data
NGS (RNA-seq) for COVID-19
Access criteria
Unrestricted-access
Total data volume
6.3 MB
File formats
  • CSV
  • ZIP
Research
hum0343
Date published
2022-05-26
Date modified
2022-05-26
Secondary ID
hum0343.v1.count.v1

Unrestricted-access files linked to this dataset

FileLabelSizeCopy URL
JCTF_bulkRNAseq_sample_info_202205.csvSample Information12.7 KB
RNAseq_TPM.zip6.3 MB

Analysis method

RNA-seq

Materials and participants
COVID-19 (ICD-10: U071): 473 cases
  • Health status
    Affected
  • Subject count
    473 (Individual)
Disease
COVID-19 (U071)
Sample description
RNAs extracted from peripheral blood cells
  • Tissue
    Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
NEBNext Poly(A) mRNA Magnetic Isolation Module
NEBNext Ultra Directional RNA Library Prep Kit for Illumina
Fragmentation
Incubation in the buffer containing Mg2+ at 94°C for 15 minutes
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
100 bp
Reference genome
GRCh38
Mapping
Adapter removal: Trimmomatic (v0.39)
Alignment: STAR (v2.7.9a)
Annotation: GENCODE v30
QC and filtering
median transcripts per kilobase million (TPM) > 10
Analysis method
Gene level quantification and normalization: RSEM (v1.3.3)
Gene count
5991
Processed data type
Count data