Dataset ID
NHA000168
- Type of data
- NGS (RNA-seq) for COVID-19
- Access criteria
- Unrestricted-access
- Total data volume
- 6.3 MB
- File formats
- CSV
- ZIP
- Research
- hum0343
- Date published
- 2022-05-26
- Date modified
- 2022-05-26
- Secondary ID
- hum0343.v1.count.v1
Unrestricted-access files linked to this dataset
| File | Label | Size | Copy URL |
|---|---|---|---|
| JCTF_ | Sample Information | 12.7 KB | |
| RNAseq_ | 6.3 MB |
Analysis method
RNA-seq
- Materials and participants
- COVID-19 (ICD-10: U071): 473 cases
- Health statusAffected
- Subject count473 (Individual)
- Disease
- COVID-19 (U071)
- Sample description
- RNAs extracted from peripheral blood cells
- TissuePeripheral blood
- Tumor / normalNormal
- Sample provider
- N/A
- Experimental method
- RNA-seq
- Target
- N/A
- Reagent kit
- NEBNext Poly(A) mRNA Magnetic Isolation Module
NEBNext Ultra Directional RNA Library Prep Kit for Illumina - Fragmentation
- Incubation in the buffer containing Mg2+ at 94°C for 15 minutes
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- 100 bp
- Reference genome
- GRCh38
- Mapping
- Adapter removal: Trimmomatic (v0.39)
Alignment: STAR (v2.7.9a)
Annotation: GENCODE v30 - QC and filtering
- median transcripts per kilobase million (TPM) > 10
- Analysis method
- Gene level quantification and normalization: RSEM (v1.3.3)
- Gene count
- 5991
- Processed data type
- Count data
- Data use policy
- NBDC data sharing policy (JGAP000001)